摘要
为了解悬浮物胁迫对菲律宾蛤仔生理状况的影响,为进一步评估海洋工程对养殖生物的损害情况提供参考依据,采用酶试剂盒方法,在实验室内研究了不同质量浓度的悬浮物胁迫对菲律宾蛤仔鳃丝和内脏团超氧化物歧化酶活力的影响。悬浮物质量浓度分别设置为0(对照)、100mg/L、1000mg/L和10000mg/L,在装有22L海水的水族箱中进行试验,水温(18.2±0.4)℃,盐度31±1,pH8.2±0.1,24h连续充气,每日全量换水一次,每4d采样一次,共进行24d。结果显示,悬浮物胁迫下,蛤仔以生理应激反应来协调适应外界不利环境,适应性诱导鳃丝和内脏团超氧化物歧化酶活力,但在长时间高质量浓度悬浮物胁迫下,显著抑制蛤仔内脏团超氧化物歧化酶活力(P<0.05),悬浮物与内脏团超氧化物歧化酶活力呈一定的剂量效应,而鳃丝超氧化物歧化酶活力对悬浮物胁迫的适应调节反应更迅速。
In order to understand the effects of suspended solids concentration on physiology of Manila clam Ruditapes philippinarum, and to assess damage of marine engineering to cultured organisms, changes in activities of superoxide dismutase（SOD） in the visceral mass and gill filament of Manila clam were studied in different concentrations of suspended substances（0, 100, 1000 and 10 000 mg/L）. Samples were collected at Days 0, 4, 8, 12, 16, 20 and 24, and stored at --80 ℃ until enzymes were assayed. The experimental condition was kept at water temperature of （18. 2±0. 4）, pH 8. 2±0. 1 and salinity（31±1.0）. The shellfish was fed algae Chlorella sp. and Chaetoceros sp twice a day. The results showed the clam generated physiological and biochemical stress response to coordinate to adapt to the suspended substance environment. SOD activity in gill filament and visceral were induced by an adapted response. During long time and high concentration of suspended solids stress, visceral SOD activity was significantly inhibited（P〉0.05） at days 24,and produced a dose-response. For gill filament and visceral SOD activity, the former had more rapid adaptation to adjust the reaction. The findings have a scientific significance and practical value for environmental rehabilitation and compensation, which are affectd by marine engineering.
出处
《水产科学》
CAS
北大核心
2013年第6期328-332,共5页
Fisheries Science
基金
辽宁省海洋与渔业厅科研计划项目(2011009
201208)
