天山雪莲SikGLP基因的克隆及表达分析

Cloning and Expression Analysis of SikGLP Gene in Sasussured involucrata Kar.et Kir

从天山雪莲cDNA文库中分析得到一个类萌发素蛋白基因序列,命名为SikGLP。生物信息学分析表明,该基因包含1个633 bp的完整开放阅读框,编码210个氨基酸。SikGLP分子量为21.6 kDa,理论等电点是6.81,并且是具有信号肽的疏水性蛋白。氨基酸多序列比对发现GLP序列保守性较高,具有的GLP的典型特征。17个物种间的系统发育树可以看出与葡萄GLP进化关系最近。Real time-PCR检测表明,SikGLP基因受低温、甲基紫精和PEG诱导表达,可能在胁迫初期对提高植物防御起作用。

A novel germin-like protein gene clone was analyzed from a cDNA library of Sasussured involucrate, named SikGLP. Bioinformatics analysis showed that the SikGLP gene contains a 633 bp open reading frame encoding 210 amino acids with a theoretical molecular mass of 21.6 kDa and an isoelectric point of 6.81. SikGLP was a hydrophobic protein and contained a signal peptide. Multiple sequence alignment of GLP proteins revealed that the GLP gene shares high identities in sequence, and possesses typical characteristics of plant germin-like proteins. Phylogenetic analysis among 17 species indicated that SikGLP was clustered with Vitis vinifera. Real time-PCR analysis showed that the expression of SikGLP gene by low temperature, MV and PEG stresses. It may plays a role in improving resistance capacity of early stress.