摘要
目的:优选麻芥平喘巴布剂的有效组分提取工艺。方法:以盐酸伪麻黄碱、苦杏仁苷的提取率和透过率为综合评价指标,采用正交试验优选提取工艺,提取物用Franz扩散池法进行体外透皮试验,HPLC测定伪麻黄碱、苦杏仁苷含量。结果:麻芥平喘有效组分的最佳提取工艺为加6倍量80%乙醇回流提取2次,每次1.5 h。结论:结合药效学指标优选的提取工艺符合实际应用需求,为麻芥平喘巴布剂的临床推广提供依据。
Objective： To optimize extraction technology of effective ingredients from Majie Pingchuan cataplasma.Method： With extraction rate and transmittance of amygdalin and pseudoephedrine hydrochloride as comprehensive evaluation index,extraction technology was optimized by orthogonal test,transdermal test of extract was adopted by Franz diffusion cell,the contents of amygdalin and pseudoephedrine hydrochloride were determined by HPLC.Result： Optimum extraction process was as following： reflux extracted 2 times with 6-fold the amount of 80% ethanol,1.5 h per time.Conclusion： Optimized technology met actual application requirements by combining with pharmacodynamic index,it could provide basis for clinical practice of Majie Pingchuan cataplasma.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第15期1-4,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技重大专项“重大新药创制”项目(2009ZX09103201)
北京中医药大学经典方剂的应用基础研究创新团队(0100603010)
关键词
麻芥平喘巴布剂
有效组分
提取工艺
伪麻黄碱
苦杏仁苷
透皮试验
Majie Pingchuan cataplasma
effective ingredients
extraction process
pseudoephedrine
amygdalin
transdermal test
