薄壳山核桃试管离体培养中不定芽诱导及增殖技术的研究

Adventitious bud induction and proliferation of Carya illinoensis in vitro culture

以薄壳山核桃实生幼苗具腋芽茎段为外植体,进行试管离体培养,以期研究其不定芽诱导及增殖技术。试验结果表明,在温度为28℃,光照度1 500 lx,光照时间为14 h/d的培养条件下,以MS+6-BA 4.0 mg/L+IBA 0.01mg/L为组成的培养基较适宜诱导供试外植体上不定芽的发生,诱导率达87%;以1/2MS+6-BA 2.0 mg/L+IBA0.1 mg/L为组成的培养基较适宜进行芽苗增殖培养,增殖系数为5.1。

An experiment was conducted on Carya illinoensisin vitroculture with the nodal stem segments excised from the newly-grown shoots of the seedlings,aiming to approach the techniques of adventitious bud induction and proliferation.Results showed that the modified MS supplemented with 6-BA 4.0 mg/L＋IBA 0.01 mg/L was optimal for the adventitious buds induction,with a maximum induction up to 87%,while the modified 1/2MS with 6-BA 2.0 mg/L＋IBA 0.1 mg/L was suitable for tube-shoot multiplication and the multiplication factor was 5.1.The above-mentioned culture was maintained at a constant temperature of 28 ℃,with 1 500 lx illumination and a 14L∶10D photoperiod.