SLE患者血清可溶性肿瘤坏死因子受体水平及意义

Significance of serum soluble tumor necrosis factor receptor levels in patients with systemic lupus erythematosus

目的探讨系统性红斑狼疮 (SLE)与可溶性肿瘤坏死因子受体 (sTNF R)的关系。方法利用双抗体夹心ELISA法检测了活动期SLE患者35例,稳定期患者25例及健康对照40例血清中sTNF RI和sTNF RII的水平。结果患者血清sTNF RI和sTNF RII的水平分别为(2.34±0.76)μg/L和(4.33±1.15)μg/L ;健康对照组分别为(1.09±0.11)μg/L和(2.05±0.29)μg/L ,患者明显高于健康对照组(P<0.01)。活动期SLE患者血清sTNF RI和sTNF RII水平分别为(2.93±0.32)μg/L和(5.19±0.53)μg/L ,明显高于稳定期SLE组分别为(1.46±0.15)μg/L和(3.04±0.28)μg/L(P∨0.01)。稳定期也明显高于健康对照组(P<0.01)。在SLE患者组中 ,血清sTNF RI和sTNF RII的水平与疾病活动积分呈显著的正相关(相关系数分别为0.76和0.69)(P<0.01) ;与抗ds DNA抗体水平亦呈显著的正相关(相关系数分别为0.67和0.58)(P<0.01) ;与补体C3的水平呈显著的负相关(相关系数分别为 -0.62和 -0.84)(P<0.01)。结论SLE患者血清sTNF RI和sTNFRII的水平明显增高 ,且与疾病的活动度呈显著正相关 ,这对于SLE的诊断及监测疾病的活动性 ,以及患者的判断预后可能是一种有用的实验室指标。

Aim To explore the relationship between sTNF R and SLE. Method The serum sTNF RI and sTNF RII levels from SLE patients(35 cases at active stage, 25 cases at inactive stage) and 40 healthy subjects were detected by double antibody sandwich ELISA. Results Both sTNF RI and sTNF RII levels in the patients were markedly higher than in the control group(P<0.01),they were respectively(2.34±0.76)μg/L and(4.33±1.15)μg/L for the patients,and(1.09±0.29)μg/L and (2.05±0.29)μg/L for the control group .Two serum sTNF R levels(2.93μg/L ±0.32μg/L and 5.19 μg/L±0.53μg/L) in active patients were obviously higher than those(1.46 μg/L ±0.28 μg/L and 3.04 μg/L ±0.53μg/L)in inactive patients(P<0.01). Moreover both sTNF RI and sTNF RII levels in inactive patients were higher than those in control group(P<0.01).So far as the SLE patients are concerned,two serum TNF R levels were positively correlated with the degree of the patient's condition, with correlation coefficients of 0.76 and 0.69, respectively(P<0.01) and also with anti ds DNA antibody levels(r=0.67 and 0.58, respectively)(P<0.01), however, were negatively correlated with the levels of complement component C3 level(r= 0.62 and 0.84 respectively)(P<0.01). Conclusion The results above showed that serum sTNF RI and sTNF RII levels were greatly increased in the SLE patients. Thus the detection of serum TNF RI and TNF RII levels could be a useful laboratory parameter for diagnosis of SLE, monitoring patient's condition and assessment of prognosis.