补骨脂4种组分对LPS诱导的RAW 264.7细胞炎症因子的影响

Inhibition of Inflammatory Cytokines in the LPS -induced RAW264.7 Cells by Four Components from Fructus Psoraleae

目的探讨补骨脂4种组分补骨脂素、corylifol A、新补骨脂异黄酮、补骨脂酚对巨噬细胞RAW264.7细胞炎症因子的影响。方法 ELISA法测定补骨脂4种组分对LPS刺激RAW264.7细胞TNF-a,IL-1β,IL-6分泌的影响及雌激素受体抑制剂(ICI 182.170)的拮抗作用。结果与正常对照组比较,脂多糖(LPS)组可以显著升高RAW264.7细胞表达TNF-α,IL-1β,IL-6(P<0.05);corylifol A、补骨脂酚在10μmol.L-1浓度下能显著降低TNF-α的分泌(P<0.05);补骨脂素、corylifol A、新补骨脂异黄酮在10μmol.L-1浓度下能显著降低IL-1β的分泌(P<0.05);corylifol A、新补骨脂异黄酮、补骨脂酚在10μmol.L-1浓度下显著降低IL-6的分泌。补骨脂4种组分的抗炎作用与补骨脂雌激素样活性无关。结论补骨脂4种不同组分对LPS刺激引起的炎症因子的释放有抑制作用,其抗炎作用与雌激素受体样活性无关。

Objective To explore the effects of four components of psoralen,corylifol A,neobavaisoflavone,bakuchiol from Fructus Psoraleae on the inflammatory cytokines in LPS-induced RAW264.7 cells.Methods ELISA was adopted to examine the effect of the four components on the secretion of inflammatory cytokines of TNF-α,IL-1β,IL-6,and on counteracting the inhibition of ICI182.170 by estrogen receptor.Results Compared with the normal control group,TNF-α,IL-1β and IL-6 levels in RAW264.7 cells were significantly increased by LPS（P 0.05）,TNF-α level was significantly decreased by corylifol A,and bakuchiol at 10 μmol.L-1,and IL-1β secretion was significantly decreased by psoralen,corylifol A and neobavaisoflavone（P 0.05）at 10 μmol.L-1.Simultaneously,IL-6 expression was also decreased by corylifol A neobavaisoflavone and bakuchiol（P 0.05）at 10 μmol.L-1.The anti-inflammatory action of the four components from Fructus Psoraleae had no correction with estrogen-like activity of Fructus Psoraleae.Conclusion The four components from Fructus Psoraleae have the inhibitory effect on inflammatory cytokines release in the LPS-induced RAW264.7 cells,and the effect has no correlation with the estrogen receptor.