幽门螺杆菌根除前后胃癌组织中MUC5AC表达的变化

目的调查幽门螺杆菌（Hp）根除前后的胃癌组织中MUC5AC表达的变化。方法采用Western Blot 和 PCR RT-PCR方法检测根除Hp前后的胃癌组织中MUC5AC蛋白及mRNA的相对含量。以Hp阴性的胃癌组织作为对照组。结果胃癌组织中MUC5AC蛋白及mRNA的表达在根除Hp后较根除前明显增高（P〈0.05），但仍然明显低于对照组。Hp阳性胃癌组织中MUC5AC的表达明显低于Hp阴性胃癌组织的表达；转移淋巴结数目大于5的Hp阳性胃癌组织中的MUC5AC相对含量明显低于Hp阴性胃癌组织。结论胃癌组织中Hp感染与MUC5AC的降低相关，但是它可能不是胃癌组织中MUC5AC减少的唯一因素，MUC5AC的减少可能只是Hp引起胃癌的一个中间环节，但可能是胃癌发生和进展的一个重要因素。

Objective To investigate MUC5AC expression in gastric cancer before and after Hp eradication. Methods The MUC5AC protein and mRNA were detected by Western Blot and real time PCR Protocols in gastric cancer before and after Hp eradication. Gastric cancer without Hp infection served as the control group. Results The MUC5AC protein and mRNA expression in gastric cancer after Hp eradication was more significantly increased than that before Hp eradication（P5 in the Hp positive group was significantly lower than that seen for the Hp negative group. Conclusions The Hp infection was correlated with a decrease in MUC5AC amount in gastric cancer,but it might not be the only factor contributing to the decreased MUC5AC expression in gastric cancer, the reduction of the MUC5AC might just be one of the middle links of which Hp causes gastric adenocarcinoma and an important factor in the progression of gastric adenocarcinoma or malignant transformation.