期刊文献+

釉基质蛋白对人牙周膜细胞群在牙骨质上附着与增殖的影响

Effects of enamel matrix protein on the attachment and proliferation of human periodontal ligament cell populations to root cementum chips
下载PDF
导出
摘要 目的研究釉基质蛋白(emdogain,EMD)对体外培养的人牙周膜细胞群(human periodontal ligament cellpopulations,hPDLPs)在牙骨质片上附着、增殖的影响,为EMD联合hPDLPs应用于牙周组织缺损修复提供实验依据。方法采用组织块法分离培养hPDLPs,制备人牙骨质片,实验组用含100 mg/mL EMD的培养液包被处理牙骨质片,对照组用不含EMD的培养液处理。分别于细胞接种牙骨质片上16 h(观察附着情况)和72 h(观察增殖情况),用噻唑蓝比色法检测牙骨质片上的细胞数;同时,用扫描电镜观察牙骨质片上的细胞数。结果噻唑蓝比色法检测,实验组细胞附着数(t=-3.318,P=0.008)、增殖数(t=-6.499,P<0.001)明显多于对照组,两组之间差异有统计学意义。扫描电镜观察计数,实验组hPDLPs附着数(t=8.001,P<0.001)、增殖数(t=13.046,P<0.001)较对照组多,差异有统计学意义。结论 EMD可促进hPDLPs在牙骨质表面的附着和增殖,提示EMD和hPDLPs可联合应用修复牙周组织缺损。 ObjectiveTo observe the effects of enamel matrix protein(EMB) on the attachment and proliferation of human periodontal ligament cell populations(hPDLPs) to root cementum chips in vitro.MethodsCementum chips obtained from the tooth roots were divided into 2 groups.One was experiment group conditioned with EMD,and the other was control group conditioned without EMD.hPDLPs attachment and proliferation were quantified by MTT assay and scanning election microscope at 16 or 72 h.ResultsMTT assay indicated that the number of adhered(t=-3.318,P=0.008) and proliferated(t=-6.499,P0.001) cells on the experiment cementum chips was significantly greater than that on the control.The scanning electron microscope assay indicated that the number of the hPDLPs on the expriment cementum chips was significantly greater than that on the control.ConclusionEMD could promote the attachment and proliferation of hPDLPs on the root cementum chips.It would be helpful for the reconstruction of periodontal tissue defects with EMD combined with hPDLPs.
出处 《广东牙病防治》 2013年第7期344-347,共4页 Journal of Dental Prevention and Treatment
基金 广东省科技计划项目(2006B35801010)
关键词 釉基质蛋白 牙周膜 细胞粘附 细胞增殖 Enamel matrix protein Periodontal ligament Cell adhesion Cell proliferation
  • 引文网络
  • 相关文献

参考文献11

  • 1Seo BM, Miura M, Grronthos S, et al. Investigation of multipotentpostnanal stem cells from human periodontal ligament[ J]. Lancet,2004,364(9429) : 149-155.
  • 2刘娟,赵红宇,轩东英,谢宝仪,章锦才.人牙周膜细胞群多向分化潜能的实验研究[J].华西口腔医学杂志,2010,28(2):185-189. 被引量:31
  • 3Venezia E, GoldsteinM, Boyan BD, et al. The use of enamel matrixderivative in the treatment of periodontal defects : A literature reviewand meta-analysis. Crit Rew Oral Biol Med, 2004,15(6) :382-387.
  • 4Davenport DR,Mailhot JM, Wataha JC,et al. Effects of enamel ma-trix protein application on the viability,proliferation,and attachmentof human periodontal ligament fibroblasts to diseased root surfaces invitro[ J] . J Clin Periodontol, 2003 ,30(2) :125-131.
  • 5张丽青,万玲,吴织芬.釉基质蛋白对牙周膜细胞增殖和碱性磷酸酶活性的影响[J].牙体牙髓牙周病学杂志,2001,11(4):225-227. 被引量:7
  • 6Hatfield CG,Baumhammers A. Cytotoxic effects of periodontally in-volved surfaces of human teeth[ J]. Arch Oral Biol,1971,16(3 ):465 468.
  • 7Haase HR, Bartold PM. Enamel matrix derivative induces matrixsynthesis by cultured human periodontal fibroblast cells [ J] . J Peri-odontol, 2001,72(3) :341-348.
  • 8Rodrigues TL, Marchesan JT, Coletta RD, et al. Effects of enamelmatrix derivative and transforming growth factor -pi on human perio-dontal ligament fibroblasts [ J]. J Clin Periodontol,2007 , 34 ( 6 ):514 -522.
  • 9Lyngstadaas SP, Lundberg E, Ekdahl H, et al. Autocrine growthfactors in human periodontal ligament cells cultured on enamel matrixderivative[ J]. J Clin Periodontol’ 2001,28(2) :181-186.
  • 10薛瑾,刘宏伟.釉基质蛋白与不同化学方式联合处理根面对牙周膜细胞附着、增殖的影响[J].牙体牙髓牙周病学杂志,2009,19(8):441-445. 被引量:4

二级参考文献35

  • 1吴军正,司徒镇强,陈建元,王为,刘斌.体外培养的人牙龈牙周膜牙髓纤维细胞生长及形态特点[J].实用口腔医学杂志,1993,9(4):227-229. 被引量:42
  • 2贾惠梅,欧阳翔英,曹采方.釉基质蛋白对牙周膜细胞在牙骨质根面上生长的影响[J].中华口腔医学杂志,2006,41(2):74-76. 被引量:8
  • 3史俊南,吴织芬,董广英,王勤涛.骨形成蛋白对牙周膜细胞增殖和碱性磷酸酶活性的影响[J].牙体牙髓牙周病学杂志,1996,6(3):153-155. 被引量:19
  • 4万玲,吴织芬,周以钧,刘斌.纤维结合蛋白对牙周韧带细胞中细胞外基质合成的影响[J].中华口腔医学杂志,1996,31(6):348-350. 被引量:7
  • 5束蓉.釉基质蛋白诱导牙周组织再生的实验研究[M].上海:上海第二医科大学口腔医学院,1999..
  • 6Lang H, Scher N, Nolden R. Attachment formation following replantation of cultured cells into periodontal defects - a study in minipigs [ J ]. Dent Res, 1998,77 ( 2 ) :293 - 405.
  • 7Hatfield CG, Baumhammers A. Cytotoxic effects of periodontally surface involved surfaces of human teeth [ J ]. Arch Oral Biol, 1971 (4), 16:465 - 468.
  • 8Gestrelius S,Andersson C , Lidstrom D, et al. In vitro studies on periodontal ligament cells and enamel matrix derivative [ J ]. J Clin Periodontol,1997,24(9 Pt2) :685 -692.
  • 9Vennzia E, Goldstein M, Boyan BD, et al. The use of enamel matrix derivative in the treatment of periodontal defects : A literature review and meta -analysis[ J]. Crit Rev Oral Biol Med, 2004, 15(6) :382 -402.
  • 10Trombelli L, Scabbia A, Scapoli C, et al. Clinical effect of tetracy- cline demineralization and fibrin -frbronectin sealing system application on healing response following flap debridement surgery [ J ]. Periodontol , 1996,67 (7) :668 - 693.

共引文献46

;
使用帮助 返回顶部