假丝酵母99-125发酵生产脂肪酶工艺研究

Study on fermentation technology of lipase production with Candida sp.99-125

对假丝酵母99-125发酵生产脂肪酶工艺进行了研究,实现了5m3发酵罐的稳定生产,确定最佳通气比(v/v)和搅拌转速分别为0.8∶1和122r/min,发酵后期补入0.5%的豆油对产酶有促进作用,在103h达到最高酶活为7 240U/mL,用3%的大豆分离蛋白和2%的大豆生物素组合替代豆粕作为氮源,可以提高发酵活力12%,达到8 179U/mL,发酵残渣量减少20%,最终罐批获得的酶活总量提高了近15%。

The fermentation technology of Lipase product with cand vestigated. Steadily productio tilatory capacity was 0.8 ： 1, n of lipase in 5 m3 fermentor was achieved. speed of agitator was 122 rpm/min. At 1 ida sp. 99-125 was inMake sure the best venater period of fermentation, adding 0.5% soybean oil promote lipase secretion, the final lipase activity reached 7 240 U/mL at 103 h; Used 3% soy source taking the place of bean pulp the fermentation residual reduced 20 bean isolate protein and 2 %soybean bio-nutrient as nitrogen , could rise lipase activity 12%, the final gross of lipase rised reached 8 179 U/mL, and nearly 15 %.