水蛭的湿法超微粉碎提取及其工艺优化

Optimization of wet ultrafine grinding technology for Hirudo extraction

目的优选湿法超微粉碎提取水蛭的最佳工艺。方法在单因素试验基础上,以料液比、浸泡时间、提取温度和粉碎时间为自变量,根据Box-Behnken原理采用4因素3水平的响应面分析法,以抗凝血酶活性为响应值,对湿法超微粉碎提取条件进行优化。结果湿法超微粉碎提取水蛭的最佳工艺条件:料液比为1∶12,浸泡时间为4.9 h,提取温度为6.5℃,粉碎时间为12 min,其抗凝血酶活性达到20.13 U/g。结论湿法超微粉碎提取技术适用于水蛭的提取,本实验优选的提取工艺稳定可行,适于进一步推广应用。

Objective To study the optimal extraction for Hirudo by wet ultrafine grinding technology. Methods On the basis of single-factor experiments, the response surface analysis was made for exploring the optimal extraction conditions by wet ultrafine grinding technology for Hirudo, with solid-liquid ratio, soaking time, extraction temperature, and grinding time as independent variable and the anticoagulant activity as the response value. According to Box-Behnken principles, the response surface analysis method with four factors and three levels was used. Results The optimum extraction condition for Hirudo by wet ultrafine grinding technology was as follows： solid-liquid ratio 1 ： 12, soaking time 4.9 h, extraction temperature 6.5 ℃, grinding time 12 min; The anticoagulant activity could reach 20.13 U/g. Conclusion The extraction by wet ultrafine grinding technology for Chinese materia medica Hirudo is stable and feasible, and it could be further promoted.