纳洛酮对缺血再灌注心肌c-fos基因表达的影响

Effects of Naloxone on Expression of c-fos Gene during Global Myocardial Ischemia-reperfusion

目的 :研究纳洛酮对心肌缺血再灌注损伤的影响 ,从分子水平探讨心肌缺血再灌注损伤的保护机制。方法 :30只大白兔随机分为三组 :(1)对照组 ;(2 )心肌缺血 30分钟再灌 30分钟组 ;(3)静注纳洛酮 10分钟后缺血 30分钟再灌 30分钟组。提取心肌总RNA与经同位素标记的c foscDNA探针进行分子杂交并自显影 ,测定c fos基因mR NA水平。结果 :纳洛酮能显著抑制心肌缺血再灌注时c fos基因的表达。结论 :纳洛酮可能具有全心缺血再灌注损伤的分子保护作用。

Objective:To observe the effects of naloxone on expression of c fos gene during global myocardial ischemia reperfusion.Methods:30 rabbits were randomly divided into control group,IR group(ischemic 30min reperfusion 30min) and NAL group (naloxone was injected before IR).Total cellular RNA was isolated after 30min of ischemia and reperfusion.Optical density(OD) value was measured.Results:OD values decreased more in NAL group than that in IR group.Conclusion:Naloxone depresses effectively the expression of c fos gene.In the molecular modulation,naloxone may protect myocardium from ischemia reperfusion injury.