摘要
目的 研究抗真菌生药活性成分对真菌细胞遗传物质的影响 ,以期发现其抗真菌的作用机理。方法 把对照组及用药组真菌细胞进行激光扫描共聚焦显微镜观察并作细胞扫描图像分析 ,从而定量描述细胞形状、面积和DNA ,RNA含量。结果 用药组细胞形态及DNA ,RNA含量发生不同变化。结论 澳洲茄胺、紫檀和白鲜碱直接或间接影响了真菌细胞遗传物质的正常合成以至不能完成正常细胞周期 。
AIM To investigate the antifungal effect of three natural products on the genetic substance of Saccharomyces cerevisiae GL7 and Prototheca wickerhamii . METHODS The normal and treated cells were observed by confocal laser scanning microscope (CLSM) and image analysis to quantitatively described the cell morphology, area, DNA and RNA content. RESULTS The morphology, area, DNA and RNA contents were changed greatly in the treated cells. CONCLUSION Solasodine, 4′ hydroxy 3,5 dimethoxystilbene and dictamnine directly or indirectly interfered the synthesis and function of genetic substance in S.cerevisiae and P.wickerhamii .
出处
《药学学报》
CAS
CSCD
北大核心
2000年第11期860-863,共4页
Acta Pharmaceutica Sinica
基金
中华医学基金专项人才基金"
"跨世纪优秀人才基金"
"国家杰出青年基金"
关键词
抗真菌生药
细胞遗传物质
LSCM
DNA
RNA
confocal laser scanning microscope
solasodine
4′ hydroxy 3,5 dimethoxystilbene
dictamnine
Saccharomyces cerevisiae GL7
Prototheca wickerhamii
genetic substa