海洋活性物质2,3-吲哚醌对雄性大鼠生殖系统的作用研究

The study of 2,3-indolinedione in reproductive system in male rat

目的探讨2,3-吲哚醌对环磷酰胺诱导的大鼠少弱精症的作用。方法模型组与低中高治疗组连续腹腔注射环磷酰胺(30 mg/kgd)5天,建立大鼠少弱精模型后,正常对照组每天生理盐水灌胃,模型组每天1.25%西黄耆胶水溶液灌胃,低、中、高剂量组分别给予2,3-吲哚醌30 mg/kg、150 mg/kg、300 mg/kg,持续35天。普通光学显微镜观察附睾精子数目、精子活力;放射免疫法检测血清中睾酮(T)、促黄体激素浓度(LH)、促卵泡激素(FSH)含量;HE染色观察生精小管层数;测定睾丸、胸腺、肝、脾重量。结果各剂量治疗组与模型组比较,精子数目增加(P<0.05),a级、a+b级精子比率上升(P<0.05),血清促卵泡刺激素(FSH)下降(P<0.05),生精小管层数增加(P<0.05)。结论 2,3-吲哚醌治疗对环磷酰胺诱导的大鼠少弱精症具有改善作用。

Objective：To study the effect of 2,3-indolinedione（ISA） in the oligozoospermia induced by cyclophosphamide（CP） in rat.Methods：The rat in model group,low-dose,mid-dose and high-dose treatment group were given CP（30 mg/kg·d） through intraperitoneal injection for 5 days,and then give control group normal saline,model group tragacanth（1.25%） and low-dose ISA（30mg/kg）,mid-dose ISA（150mg/kg） and high-dose treatment group ISA（300mg/kg） by intragastric administration for 35 days.Measure the sperm mobility rate,sperm counts,serum testosterone（T）,follicle-stimulating hormone（FSH）,luteinizing hormone（LH） levels,seminiferous tubule layers and the mass of testis,thymus,liver and spleen.Results：Compared to Model group,three treatment groups showed obvious increase of the sperm counts（P0.05）,sperm mobility（P0.05） rate and seminiferous tubule layers（P0.05）,and decrease of the concentrations of follicle-stimulating hormone（FSH） levels（P0.05） in serum.The mass of testis,thymus,liver and spleen showed no obviously changes.Conclusion：2,3-indolinedione could improve the oligozoospermia induced by cyclophosphamide（CP）.