来那度胺对人宫颈癌SiHa细胞生长的影响

Effects of lenalidomide combined with cisplatin on the growth of SiHa cells

目的探讨来那度胺及其联合顺铂对体外培养的人宫颈癌SiHa细胞增殖的抑制作用。方法体外培养SiHa细胞,实验设对照组、来那度胺组、顺铂组和来那度胺+顺铂联合组,各组药物处理后,用MTT法检测细胞生长抑制率,流式细胞术检测细胞周期分布。结果 MTT法检测结果显示,来那度胺与顺铂联用后对SiHa细胞的生长抑制作用较单用顺铂强(P<0.05),但来那度胺单独作用对SiHa细胞生长的影响与对照组比较无明显差异(P>0.05);流式细胞周期分析结果显示,来那度胺作用于SiHa细胞后,与对照组相比G1期细胞百分比增高(P<0.05),与顺铂联用后细胞周期阻滞作用增强(P<0.05)。结论来那度胺与顺铂联用可增强顺铂对SiHa细胞的生长抑制作用;来那度胺作用于SiHa细胞后可将细胞周期阻滞于G1期,与顺铂联用后细胞周期阻滞作用增强。

Objective To investigate whether lenalidomide alone or in combination with cisplatin can inhibit the growth of human cervical carcinoma SiHa cells in vitro. Methods Human cervical carcinoma SiHa cell line was cultured in vitro and then treated with different concentrations of lenalidomide, cisplatin, or lenalidomide combined with cisplatin in different groups. MTT was used to determine the growth inhibition ratio; flow cytometry was used to determine the effect of the drugs on cell cycle. Results The result of MTT showed that lenalidomide in combination with cisplatin enhanced the inhibitory effect of cisplatin on cell growth （P〈0. 05）, but there was no significant difference between the lenalidomide group and control group （P〉 0.05）. The result of flow cytometry showed that the percentage of G1 phase of lenalidomide group was significantly higher than that of control group （P〈0.05） and that the effect on cell cycle arrest could be enhanced when lenalidomide was used in combination with cisplatin （P〈0. 05）. Conclusion Lenalidomide combined with cisplatin can enhance cisplatin＇s suppression on the growth of SiHa cells. Lenalidomide can arrest cell cycle in G1 phase, and when combined with cisplatin it has the synergistic effect on cell cycle arrest.