碱性成纤维细胞生长因子对庆大霉素椭圆囊毛细胞毒性的拮抗作用

THE ANTITOXIC EFFECT OF bFGF ON UTRICULAR HAIR CELLS INJURYED BY GENTAMICIN

本研究采用豚鼠椭圆囊培养的方法观察了碱性成纤维细胞生长因子 (bFGF)对庆大霉素所致离体椭圆囊毛细胞毒性的拮抗作用。用庆大霉素造成培养椭圆囊毛细胞的损伤 ,实验组椭圆囊用含bFGF培养液培养 ,对照组培养液则不含bFGF。所有椭圆囊均行扫描电镜检查并行毛细胞计数。实验组毛细胞的存活数目显著高于对照组 (P <0 .0 5 )。bFGF对庆大霉素所致离体椭圆囊毛细胞毒性有拮抗作用。

This study is to investigate the antitoxic effect of basic Fibroblast Growth Factor(bFGF) on guinea pig utricular hair cells destroyed by gentamicin in vitro. Gentamicin was used to destroy guinea pig utricular hair cells in vitro . The cultures of experimental groups were supplemented with bFGF. The cultures of control group were not supplemented with bFGF. All utricles were observed by scanning electronic microscope, and the number of survival utricular hair cells was accounted. A significantly higher number of survival utricular hair cells was seen in experimental group ( P <0.05), as compared with control group. bFGF has antitoxic effect on guinea pig utricular hair cells injured by gentamicin in vitro.