摘要
使用普通PCR和荧光定量PCR方法对珠江口典型水产养殖区水和沉积物中3种磺胺类、7种四环素类、1种喹诺酮类抗生素抗性基因(antibiotic resistance genes,ARGs)和1种整合子基因进行了定性和定量研究.结果表明,除tetW外,所有其他ARGs在珠江口养殖区中均被检出,其中sul1、sul2和int1的检出率为100%.相同养殖模式下,养殖时间越长ARGs的相对含量越高,表明ARGs具有累积效应;不同养殖模式池塘中ARGs的含量存在差异,表明养殖模式可能影响ARGs的含量与分布.int1相对含量与sul1和ARGs总量之间均存在显著相关性(P<0.05),表明int1在ARGs的水平传播中起着非常重要的作用.此外,珠江口水产养殖区沉积物中抗生素浓度与ARGs总量存在显著相关性(P<0.05),说明水产养殖区中抗生素残留是诱导养殖区ARGs的主要因素.
Traditional and quantitative PCR techniques were used to determine the occurrence and quantities of ARGs, including three types of genes resistant to sulfonamide, seven for tetracycline resistance and one for quinolone resistance, as well as one integron gene in typical aquaculture of the Pearl River Estuary. The results showed that all genes except for tetW were detectable in the aquaculture environment, and sull, sul2 and intl were the most frequently detected genes (detectable percentage, 100% ). Relative abundances of ARGs increased with the prolongation of rearing time under the same aquaculture pattern, suggesting a cumulative effect. Moreover, the occurrences of ARGs in the ponds were different with different aquaculture patterns, indicating that the aquaculture pattern might play an important role in the abundances and distributions of ARGs. Relative abundances of intl, as a horizontal mobile genetic element, were significantly correlated to the levels of sull and the total ARGs (P 〈 0.05). The total concentration of antibiotics exhibited a good positive correlation with the total concentration of ARGs in sediments ( P 〈 0. 05 ). All results elucidated that extensive residues of antibiotics in the aquaculture substantially increased the abundances of ARGs probably owning to the induction of horizontal gene transfer of ARGs among bacteria.
出处
《环境科学》
CAS
CSCD
北大核心
2013年第10期4073-4080,共8页
Environmental Science
基金
中国科学院-国家外国专家局创新团队国际合作伙伴计划项目(KZCX2-YW-T001)
中国科学院知识创新工程项目(KZCX2-YW-Q07)
国家自然科学基金项目(41076069,40776086)
关键词
抗生素抗性基因
普通PCR
荧光定量PCR
水
沉积物
水产养殖区
珠江口
antibiotic resistance genes
traditional PCR
real-time quantitative PCR
water
sediment
aquaculture
Pearl River Estuary