摘要
目的:探索KLK5基因作为诊断基底细胞样乳腺癌(BLBC)的分子标志和治疗靶点的可能。方法:通过包含48 804个探针的人类mRNA基因表达谱芯片比较48例各亚型乳腺癌和6例正常乳腺组织基因表达谱,并通过荧光实时定量PCR法进行结果验证,分析BLBC与其他各亚型乳腺癌基因之间的差异表达。结果:分析乳腺癌各亚型和正常乳腺组织的基因表达谱,在BLBC中有99个基因上调4倍以上(2倍以上有意义),其中KLK5基因显著上调(5倍以上),但KLK5在Luminal A、Luminal B、HER-2过表达的基因表达谱中下调超过2倍,它们之间的差异表达具有统计学意义(P<0.05);并通过RT-PCR检测得到进一步验证。结论:KLK5可以作为诊断基底细胞样乳腺癌的标志基因和治疗靶点。
Objective: To investigate the underlying role of gene KLK5 as the therapeutic tar- gets for basal-like breast carcinoma. Methods: The gene-expression profiles of 48 cases of the breast carcinoma and 6 cases of normal mammary epithelium were analysed using human mRNA genome expression profiling chip containing 48 804 probes in attempt to characterize molecular mechanism involved in the carcinogenesis of basal-like carcinoma of the breast, Real time-PCR was also used to validate results of each of the breast carcinoma subtype tissues and normal mammary epithelium tissues. Reults: The mRNA gene expression profiling analysis of breast cancer mRNA and normal mammary ductal epithelial cells genomes expression profiling chips, it showed that 99 genes in BLBC upregulates more than 4-folds (more than 2-folds are indentified as significant), of these the gene KLK5 upregulates significantly (more than 5-folds).However, the gene KLK5 in Lu- minal A,Luminal B and HER2-positive breast cancer cells downregulates more than 2-folds, the dif- ferences have statistic significance (P〈O.05). RT-PCR and signaling network were further analyzed for the molecular regulation.The result is the same. Conclusion: It was found gene KLK5 in BLBC was significantly differed with other subtypes, such result has not been reported. It was assumed that KLK5 diferentially expressed gene could be used as a novel marker and a therapeutic target for BLBC.
出处
《中国现代普通外科进展》
CAS
2013年第9期673-677,共5页
Chinese Journal of Current Advances in General Surgery
基金
国家自然科学基金(30870975
81272905)