摘要
目的:观察附子理中汤对免疫细胞因子白细胞介素-2(IL-2)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)和肿瘤坏死因子-α(TNF-α)的影响,探讨其对脾阳虚证免疫功能影响的可能机制。方法:Wistar大鼠100只随机分为对照组、模型组、附子理中汤低、中、高剂量组5组,每组20只。在模型组基础上,附子理中汤低、中、高剂量组按5,10,20 g·kg-1ig,每天1次,连续4周。检测胸腺指数、脾脏指数、IL-2,IL-6,IL-10,TNF-α含量。结果:与对照组比较,模型组胸腺指数,脾指数,IL-2,IL-6,TNF-α均降低(P<0.05或P<0.01),IL-10显著升高(P<0.01)。与模型组比较,附子理中汤中、高剂量组胸腺指数,脾指数,IL-2,IL-6,TNF-α均升高(P<0.05或P<0.01),IL-10降低(P<0.05或P<0.01)。结论:附子理中汤对细胞免疫因子产生影响,通过细胞免疫因子之间的效应可能是其改善脾阳虚大鼠免疫功能的机制之一。
Objective: To explore the effects and mechanism of Fuzi Lizhong decoction(FZLZ) on immune cell factors in rats with spleen Yang deficiency syndrome.Method: One hundred Wistar rats were randomly divided into model group,FZLZ low-dosage group,middle-dosage group,high-dosage group of FZLZ and control group.Each group included 20 rats.Low-dosage group,middle-dosage group and high-dosage group were ig given with FZLZ according to 5,10,20 g·kg-1based on model group,once a day for four weeks.Then thymus index,spleen index,the contents of interleukin(IL)-2,IL-6,IL-10 and tumor necrosis factor-α(TNF-α) were examined.Result: Compared with control group,thymus index,spleen index,the contents of IL-2,IL-6 and TNF-α in model group reduced(P < 0.05 or P < 0.01),however,the content of IL-10 increased significantly(P< 0.01).Compared with model group,thymus index,spleen index,the contents of IL-2,IL-6 and TNF-α in both middle-dosage group and high-dosage group increased(P < 0.05 or P < 0.01) while the content of IL-10 reduced(P <0.05 or P < 0.01).Conclusion: Fuzi Lizhong decoction can improve immune function of rats with spleen Yang deficiency syndrome by regulating the contents of IL-2,IL-6,IL-10 and TNF-α,which may be one of the mechanisms.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第21期179-182,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西高等学校优秀人才资助计划(桂教人〔2011〕40号)
关键词
附子理中汤
脾阳虚证
免疫
机制
Fuzi Lizhong decoction
spleen Yang deficiency syndrome
immune
mechanism