导流杂交基因芯片技术检测人乳头瘤病毒结果分析

Flow-through hybridization detection of human papillomavirus DNA microarray analysis

目的分析妇产科就诊妇女人乳头瘤病毒(HPV)感染、基因型和年龄特征的流行病学情况。方法对2008年1月—2012年12月妇产科就诊的6230例妇女进行下生殖道HPV感染分型检测,采用核酸分子快速导流杂交基因芯片技术(HybriMax)检测21种亚型,其中高危型(HR-HPV)15种,低危型(LR-HPV)6种。结果在6230例标本中,阳性标本1664(26.71%),单型感染1213例(19.47%),多型感染451例(7.24%)。感染率占前5位的亚型分别是:HPV16型335例(5.38%)、HPV52型334例(5.36%)、HPV58型243例(3.90%)、HPV6型164例(2.63%)、HPV53型152例(2.44%)。高危型HPV16、HPV52和HPV58及低危型HPV8304和HPV11检出率逐年下降;所有患者中≤20岁年龄段HPV检出率最高为52.04%。结论应用HybriMax技术检测HPV的检出率为26.71%,主要为HPV16、HPV52、HPV58、HPV6和HPV53,人群感染高峰在≤20岁年龄段。

Objective To analysis clinic analysis of human papilloma virus（HPV） infection,genotype and age characteristics of the epidemiological situation in obstetrics and gynecology.Methods From January 2008 to December 2012,6230 cases of obstetrics and gynecology clinic for lower genital tract of women infected with HPV genotyping were investigated,using nucleic acid molecules,hybridization and gene chip deflector（HybriMax） detected 21 kinds of subtypes,including 15kinds of high-risk（HR-HPV） and 6 low-risk（LR-HPV）.Results In 6230 cases of specimens,positive samples 1664（26.71%）,single infection 1213 cases（19.47%）,more than 451 cases of infection（7.24%）.Infection accounted for the top five subtypes are：HPV16 type 335 cases（5.38%）,HPV52 type 334 cases（5.36%）,HPV58 type 243 cases（3.90%）,HPV6 type 164 cases（2.63%）,and HPV53 type 152 cases（2.44%）.High-risk HPV16,HPV52 and HPV58 and HPV11low risk HPV8304 detection rate and declining;all patients aged ≤ 20 HPV detection rate up to 52.04%.Conclusion Application of HybriMax to detect HPV,the detecUon rate is 26.71%,mainly for HPV16,HPV52,HPV58,HPV6 and HPV53,people infected with the peak at age≤ 20 years of age.