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黄豆脲酶波氏法尿素测定试剂盒的研制与应用 被引量:7

DEVELOPMENT AND APPLICATION OF KIT FOR DETERMINATION OF UREA BY USING SOY BEAN UREASE-BERTHELOT METHOD
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摘要 探索了黄豆脲酶用作尿素测定工具酶的反应条件 ,配制成脲酶 - Berthelot法尿素测定试剂盒 ,并通过严格的基础和应用评价实验 ,对试剂盒的分析性能进行了客观评价。其测定线性范围上限达到 35 .70 mmol/L 尿素 ( 1 0 0 mg/d L尿素氮 ) ,平均回收率为 98.5 5 % ,精密度 ( CV% )为 0 .6 6 % ,与国家定型产品对照测定 42例临床血清尿素标本 ,两者测定结果高度相关。结果表明用黄豆脲酶替代进口巨豆脲酶研制的脲酶 - Berthelot法试剂盒分析性能优越 ,可应用于临床。 A kit for determining urea by using soy bean urease as tool enzyme was developed.A series of experiments about the properties of analysis were performed.Linearity of the kit attained rose to 35.70 mmol/L. Average recovery rate was 98.55%.Variance coefficient (CV%) was 0.66% .The control test with formal product showed that both results of determination on 42 samples were highly correlative.The kit that used soy bean urease as tool enzyme had favorable properties of analysis and could be applied to clinic treatment.
出处 《甘肃科学学报》 2000年第3期56-60,共5页 Journal of Gansu Sciences
基金 甘肃省科学技术攻关项目!( GS992 -A4 3 -0 74 )
关键词 血清 尿素测定 Berthelot法 黄豆脲酶 试剂盒 determination of serum usrea urease Berthelot method
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