胃癌及癌前病变微卫星不稳定的研究

Study of the microsatellite instability involved in human gastric carcinoma and precancerous lesion

目的 探讨胃癌及癌前病变微卫星不稳定（MSI）的存在与否及其作用.方法 利用激光显微切割技术分别收集胃癌、肠化、异型增生及正常胃黏膜,选择5个微卫星位点应用变性高效液相色谱技术检测MSI.结果 5个微卫星位点REF阳性表型中,肠化MSI为10.5％,异型增生MSI为21.12％,胃癌MSI为43.75％,正常胃黏膜无MSI.结论 激光捕获显微切割技术有效地解决了异质性问题.变性高效液相色谱技术检测MSI具有半自动化、快速、检出率高等优点.MSI从癌前到胃癌逐步增加,癌前MSI的检测对胃癌的早期诊断可能是一个潜在的预警指标.

Objective To explore whether microsatellite instability （MSI） is exsist in human gastric carcinoma and precancerous lesion.Methods The MSI was detected in gastric carcinoma and precancerous lesion by tissue laser capture microdissection and denaturing high performance liquid chromatogragh （DHPLC）.Results MSI was found in 10.5 % intestinal metaplasia,21.12 % gastric hyperplasia and 43.75 %gastric carcinoma respectively.In contrast,there was no microsatellite instability in normol gastric mucosa.It displayed accumulation and alteration from normal to cancerous tissues.Conclusion Pure tumor samples are obtained from tissue laser capture microdissection which could avoid the heteroplasmy of tissue.DHPLC is a high sensitive,fast and semiautomated method for identifying MSI.This experiment showed an increasing tendency of MSI incidence from precancerous lesion to gastric carcinoma.MSI could be used as a marker to predicate the occurrence of gastric carcinoma.