甘氨酰谷氨酰胺二肽对黄羽肉鸡肝脏脂肪代谢的影响及其机制的研究

Study on the Effects of Glycyl-Glutamine Dipeptide on the Lipid Metabolism in Liver of Yellow-feathered Broiler and Its Mechanism

选择360只1日龄优质黄羽肉鸡,随机分为4组,分别饮用凉开水配制的浓度为0、0.01、0.05和0.10mg/L的甘氨酰谷氨酰胺(Gly-Gln)二肽溶液,连续饮用41 d。用甘油三酯试剂盒测定肝脏总甘油三酯的含量。采用相对定量RT-PCR的方法来检测ACC(acetyl-CoA carboxylase)和PPARα(peroxisome proliferatoractivated receptorsα)mRNA的表达水平。试验结果表明:①Gly-Gln二肽能够呈现剂量依赖性的降低肝脏ACC mRNA的表达水平,0.10 mg/L Gly-Gln二肽处理组较对照组差异显著(P<0.05)。②Gly-Gln二肽能够显著上调PPARα基因mRNA的表达水平,其中0.05 mg/L Gly-Gln二肽处理组较对照组差异显著(P<0.05)。③Gly-Gln二肽具有提高肝脏甘油三酯含量的趋势,但差异不显著(P>0.05)。以上结果提示,Gly-Gln二肽能够通过降低肝脏甘油三酯的合成,促进代谢,从而减少脂肪沉积水平。

360 one-day old yellow-feathered broilers were randomly divided into four groups to drink with 0, 0.01, 0.05 and 0.10 mg/L glycyl-glutamine dipeptide （made with cool boiled water） respectively for 41 days. The content of TG in the liver was determined by commercial TG kit. By using semi-quantitative RT-PCR,mRNA expression level of ACC and PPAR ot were detected. The results showed that Gly-Gln dipetide dose-dependently decreased the expression level of A CC mRNA in the liver and that in 0.10 mg/L Gly-Gln dipetide group had significant difference with control group （P〈0.05）. Gly-Gln dipetide could significantly increased the expression level of PPAR ot mRNA and that in 0.05 mg/L Gly-Gln dipetide group had significant difference with control group（P〈0.05）. Gly-Gln dipetide had the trend of increasing the TG content in the liver, but the difference was not significant （P 〉0.05）. Therefore Gly-Gln dipetide could decrease the synthesis of TG in the liver and promote metabolism, so as to reduce body fat deposition.