摘要
目的:研究芒果叶醇提取物(MLE)对小鼠急性酒精肝损伤的影响.方法:18 ~22 g昆明种小鼠,雌雄各半,随机分为正常组、模型组、阳性药(护肝片1.5 g·kg-1)组,MLE高、中、低(15.0,5.0,2.5 g·kg-1)剂量组,每组10只.小鼠1次性灌胃50%乙醇(12 mL·kg-1)建立急性酒精性肝损伤模型,测定血清中丙氨酸转氨酶(ALT)、门冬氨酸转氨酶(AST)活性及甘油三酯(TG)含量,检测肝组织中总超氧化物歧化酶(T-SOD)、谷胱甘肽(GSH)与丙二醛(MDA),并进行肝组织病理切片检查.结果:与模型组比较,MLE高、中、低剂量组血清TG与肝组织MDA含量及肝损伤病理积分均明显降低(P <0.05);MLE高、中剂量组小鼠血清ALT与AST活性明显降低(P<0.05),肝组织GSH水平明显升高(P <0.05);MLE高剂量组肝组织T-SOD活性明显升高(P<0.05).结论:MLE对小鼠急性酒精肝损伤具有保护作用,可能与其抗脂质过氧化作用相关.
Objective:To observe the effect of mango leaf extract (MLE) on acute alcoholic liver injury in mice.Method:Sixty mice were randomly divided into control group,model group,positive drug group (Hugan tablet 1.5 g ·kg-1),MLE high,middle and low-dose (15.0,5.0,2.5 g ·kg-1) group,10 each group.Acute liver injury model was induced by 50% alcohol given by gavage.The levels of alanine aminotransferase (ALT),aspartate aminotransferase (AST),triglycerides (TG) in the serum and malondialdehyde (MDA),glutathione (GSH),total superoxide dismutase (T-SOD) in the liver tissue were measured.Liver index was calculated and pathological changes of the liver tissue were observed under optical microscope.Result:Compared with model group,the serum TG in MLE high,middle and low dose group significantly lowered (P 〈 0.05) ; the serum ALT,AST in MLE high,middle dose group significantly lowered (P 〈 0.05) ; T-SOD activity in MLE high dose group rose obviously (P 〈 0.05) ; GSH levels of liver tissue in MLE high and middle dose group rose obviously (P 〈 0.05) ; MDA content in liver tissue in MLE high,middle and low dose group lowered obviously (P 〈 0.05) ; the liver pathological score of MLE high,middle and low dose group lowered significantly (P 〈0.05).Conclusion:MLE has a protective effect on acute liver injury induced by alcohol in mice,which may be related to its anti-lipid peroxidation.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第22期250-253,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西科技攻关项目(桂科攻10124008-10)
广西自然科学基金项目(2010GXNSFA013194)
关键词
芒果叶
急性酒精性肝损伤
转氨酶
甘油三酯
超氧化物歧化酶
丙二醛
mango leaf
mouse
acute alcoholic liver injury
transaminase
triglycerides
superoxide dismutase
malondialdehyde