苦参碱联合顺铂对子宫颈癌SiHa细胞中TSLC1基因表达的影响

Effects of matrine combined with cisplatinum on the expression of TSLC1 gene in human cervical cancer SiHa cell

目的探讨苦参碱联合顺铂对人宫颈癌SiHa细胞肺癌肿瘤抑制因子1(TSLC1)基因的表达及SiHa细胞对顺铂敏感性的影响,以期对治疗宫颈癌提供依据。方法采用四甲基偶氮唑蓝比色法(MTT)测定不同浓度苦参碱组(50、100、150、200、250μg/ml)、顺铂组(5、10、15、20、25μg/ml)和联合组(15μg/ml顺铂联合5种浓度苦参碱)干预SiHa细胞后的抑制率;采用实时荧光定量PCR(FQ-PCR)技术检测TSLC1mRNA表达水平的变化。结果苦参碱及顺铂呈剂量依赖方式抑制SiHa细胞的生长,且随着各组浓度的增高,TSLC1 mRNA表达上调(P<0.01)。不同浓度联合组对细胞的抑制率分别为39.35%、63.51%、68.74%、72.04%和74.31%,TSLC1mRNA的表达量分别为8.59±0.01、15.33±0.01、26.38±0.07、61.34±0.01和92.33±0.01,与苦参碱组或顺铂组比较,差异均有统计学意义(P<0.05)。结论苦参碱联合顺铂抑制人宫颈癌SiHa细胞生长的机制可能与TSLC1mRNA的表达上调有关,且苦参碱能够增加SiHa细胞对顺铂的敏感性。

Objective To investigate the effects of matrine only or combined with cisplatinum （DDP） on the expression of TSLC1 gene in human cervical cancer SiHa cell and its sensitive effect on SiHa cells to cisplatin. Methods SiHa cells cultured in vitro were treated for 48 hours with matrine only, DDP only or matrine combined DDP, inhibition of cells were detected by MTT. The expression of TSLC1 mRNA were detected by FQ- PCR. Re- sults The inhibitory effect of matrine only and DDP only on the growth of SiHa cell lines was in a time dependent manner. The apoptosis rate and TSLC1 mRNA expression were obviously increased following the increasing concen- tration （P〈0.01）. When treated with matrine combined with DDP, the inhibition rate was much higher than trea- ted with matrine only or DDP only （P〈0. 05） . Conclusion Matrine and DDP could inhibit proliferation and induce apoptosis of SiHa cells, which may be related to the upregulation of TSLC1 mRNA expression as well as increase the sensitivity of Hela cells to DDP.