HLA-A、-B、-C、-DRBl和-DQB1等位基因全相合的无关供一受者对的HLA-DPA1和-DPB1基因匹配性研究

A study of HLA-DPA1 and DPB1 matching status for unrelated donor-recipient pairs matched at allele level

目的研究HLA-A、-B、-C、-DRB1和-DQB1等位基因全相合的无关供-受者对HLA-DPA1和-DPB1基因的匹配性。方法对76对HLA-A、-B、-C、-DRB1和-DQB1等位基因10／10相合的无关供-受者样本进行HLA～DPA1和-DPB1基因测序分型，从等位基因水平统计和分析HLA-DPA1和-DP通信作者：邓志辉，Emai1：zhihui-deng@sina．cn基因匹配的比例和特点。结果单个HI。A-DPA1、-DPB1基因高分辨水平完全相合的比例分别为17．1％、9．2％，完全相合的比例均较低。HI，A-DPA1等位基因半相合的比例占绝大多数（73．7％），完全不合的比例为9．2％；具有高度多态性的HLA-DPB1基因半相合的比例为57．9％，而完全不合的比例为32．9％。统计HLA-DPA1＋-DPB1等位基因的匹配率，e／4相合的比例最高（51．4％），4／4相合的比例仅为5．6％，而0／4相合的比例为8．3％。结论HLA-A、-B、-C、～DRB1和-DQB1等位基因相合的无关供-受者对HLA-DPA1和-DPB1基因匹配率尚较低，HLA-DPA1和-DPB1基因匹配程度对无关供者造血干细胞移植的影响应引起临床的重视和探讨。

Objective To analyze the status of HLA-DPA1 and DPB1 matching for unrelated donor- recipient pairs matched at high-resolution allele level for HLA-A, B, C, DRB1 and DQB1 loci. Methods A total of 76 unrelated donor-recipient pairs matching at allele level for HLA-A, B, C, DRBland DQB1 loci were subjected to HLA-DPA1 and DPB1 sequence-based typing （SBT）. HLA-DPAland DPB1 matching status at high-resolution allelic level was also analyzed. Results The allelic identity ratio for single HLA- DPA1 and DPB1 were 17. 1% and 9.2%, respectively. HLA-DPA1 and DPB1 allelic identity ratio were both very low. The majority of unrelated donor-recipient pairs （73.7%） had an incompatibility at 1 HLA- DPA1 allele, 9.2% of pairs had an incompatibility at 2 DPA1 alleles. As for the high-polymorphic HLA- DPB1 gene, 57.9% of studied donor-recipient pairs had an incompatibility at 1 HLA-DPB1 allele, almost 1/ 3 （32. 9%） of them were completely incompatible. When HLA-DPA1 and DPB1 genes were analyzed together, the donor-recipient pairs matched at 2/4 was the most common （51.4%）, 4/4 allelic complete matched pairs accounted for 5.6%, and 0/4 matched pairs accounted for 8. 3%. Conclusion Our results indicated that the ratio of HLA-DPA1 and DPB1 complete match in the unrelated donor-recipient pairs matching at allelic level for HLA-A, B, C, DRBland DQB1 loci were very low. The effect of HLA-DPA1 and DPB1 matching status on clinical unrelated stem cell transplantation still needs to be elucidated.