TSP-1在子痫前期患者血清及胎盘组织中的表达研究

Study Of Serum and Placental Tissue TSP-1 Levels in Patients with Preeclampsia

目的通过研究血小板反应蛋白(thrombospondin,TSP)-1在子痫前期(preeclampsia,PE)患者血清及胎盘组织中的表达,探讨其与子痫前期发病的关系及其临床意义。方法选取子痫前期患者30例,其中轻度子痫前期15例,重度子痫前期15例,选取同期正常孕妇15例作为对照组,采用ELISA法检测三组孕妇血清中TSP-1的表达水平;同时采用免疫组化SP法检测相应胎盘组织中的TSP-1的表达水平。结果血清表达:①轻度子痫前期与重度子痫前期分别与对照组的比较,P<0.05,有统计学意义。②轻度子痫前期与重度子痫前期患者之间比较,P>0.05,差异无统计学意义。胎盘表达:①轻度子痫前期与重度子痫前期分别与对照组的比较,P<0.05,差异有统计学意义。②轻度子痫前期与重度子痫前期患者之间比较,P>0.05,差异无统计学意义。结论血小板反应蛋白-1在子痫前期患者血清及胎盘组织中表达均较正常产妇升高,与子痫前期的发病有关,但与子痫前期的严重程度关系不大。

Objective To observe the level of TSP-1 in serum and placental tissue in patients with preeclampsia. Methods 30 patients with preeclampsia were collected.Among them, 15 cases were mild preeclampsia and 15 cases were severe preeclampsia.15 healthy pregnant women were selected as control. The serum level of TSP-1 in the three groups was measured by using enzyme-linked immunosorbent assay（ELISA）.The placental level of TSP-1 in the three groups was measured by using Immunohistochemistry（SP）. Results Serum expression：①mild preeclampsia and severe preeclampsia, respectively, compared with the control group, P〈0.05, statistically signiifcant.②mild preeclampsia and severe preeclampsia compared between patients, P〉0.05, no signiifcant difference. Placental expression：①mild preeclampsia and severe preeclampsia, respectively, compared with the control group, P〈0.05, the difference was statistically signiifcant.②mild preeclampsia and severe preeclampsia compared between patients, P〉0.05, the difference was not statistically signiifcant. Conclusion TSP-1 level was signiifcantly increased in patients with preeclampsia.However it is not directly related to the severity of preeclampsia.