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UPLC同时测定百灵安神片中7种活性成分含量 被引量:1

Simultaneous Determination of Seven Active Compounds in Bailing Anshen Tablets by UPLC
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摘要 目的建立UPLC同时测定百灵安神片中绿原酸、芒果苷、二苯乙烯苷、斯皮诺素、木犀草苷、槲皮苷、3,5-二咖啡酰奎宁酸含量的方法。方法采用Waters Acquity UPLC BEH C18(2.1 mm×100 mm,1.7μm)色谱柱;流动相:乙腈-0.1%磷酸水,梯度洗脱;流速:0.4 mL·min-1;检测波长:340 nm。结果绿原酸、芒果苷、二苯乙烯苷、斯皮诺素、木犀草苷、槲皮苷、3,5-二咖啡酰奎宁酸在检测范围内线性良好,r均>0.999 8,方法回收率分别96.3%,98.2%,100.5%,99.3%,97.7%,97.0%,96.4%。结论本方法准确可靠快捷,为全面控制百灵安神片质量提供了一种测定方法。 OBJECTIVE To established a UPLC method for determination of seven active compounds in Bailing Anshen tablets, including chlorogenic acid, mangiferin, tetrahydroxystilbene, spinosin, cynaroside, quercitrin, and 3,5-dicaffeoylquinic. METHODS The chromatographic separation performed on a C18 column and with gradient elution of acetonitrile and 0.1% aqueous phosphoric acid at a flow rate of 0.4 mL·min^-1. The detected wavelength was set at 340 nm. RESULTS The seven analytes showed good linearity (r〉0.999 8) within the test ranges. The average recoveries were 96.3%, 98.2%, 100.5%, 99.3 %, 97.7%, 97.0%, 96.4%, respectively. CONCLUSION The analytical method is simple, rapid, specific, which is useful for quality control of Bailing Anshen tablets.
出处 《中国现代应用药学》 CAS CSCD 2013年第12期1331-1333,共3页 Chinese Journal of Modern Applied Pharmacy
基金 "重大新药创制"科技重大专项(2011ZX09401-306)
关键词 超高效液相色谱法 百灵安神片 质量控制 UPLC Bailing Anshen tablets quality control
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