新型分子标记物对甲状腺滤泡型肿瘤良恶性的鉴别诊断

Application of molecular biomarkers in differential diagnosis of benign and malignant follicular thyroid tumors

收集29例甲状腺结节组织标本，提取总RNA，分别进行RT-PCR在核酸水平上检测所取甲状腺组织中4种目的基因C10rf24、DDIT3、HMGA2、TFF3的表达水平。与正常甲状腺组织相比，C10rt24mRNA在滤状腺瘤中的表达下调，在滤泡状癌中的表达上调；DDIT3和HMGA2mRNA在滤泡状腺瘤和滤泡状癌中的表达均上调，且在滤泡状癌中的表达高于滤泡状腺瘤：TFF3mRNA在滤泡状癌和滤泡状腺瘤中均下调。甲状腺肿瘤组织中C10rt24、DDIT3、HMGA2、TFF3对甲状腺滤泡状癌和滤泡状腺瘤的鉴别有一定价值．

Twenty-nine thyroid tissue samples were collected from patients with thyroid nodules. The total RNA were extracted, the gene expressions of TFF3, HMGA2, Clorf24, and DDIT3 were detected by RT-PCR. In comparison with normal thyroid tissues, the expression of Clorf24 mRNA was decreased in the follicular thyroid adenoma （ FTA ） group, but increased in the follicular thyroid carcinomas （ FTC ） group. The expressions of DDIT3 and HMGA2 mRNA were increased in both FTA and FTC groups, and were even higher in the latter. The expressions of TFF3 mRNA level were decreased in FTA and FTC. The data suggested that molecular markers Clorf24, DDIT3, HMGA2, and TFF3 in thyroid tissue seem to be helpful in the differential diagnosis between follicular adenomas and carcinomas.