东方百合“Siberia”基因组DNA提取方法比较研究

Comparative Study on Genomic DNA Extraction Methods From the Oriental Lily‘Siberia'

以东方百合"Siberia"为试材,研究比较了十二烷基硫酸钠(SDS)法、溴化十六烷基三甲基铵(CTAB)法及改良的SDS法、CTAB法对提取百合不同部位基因组DNA的效果。结果表明:利用改良的CTAB法提取百合不同部位的DNA效果最好,点样孔无杂质,且没有拖尾现象;通过增加抽提次数以及改用3MNaAc进行沉淀可以提高百合鳞茎和根部DNA的提取率,降低提取DNA的污染程度。

Taking the Oriental lily ＇Siberia＇ as material,SDS,C＇TAB, modified SDS and modified CTAB methods to extract genomic DNA were compared. The results showed that the modified CTAB method was the best method to extract DNA from lily. There were no impurities and tail in sample, the quantity and quality of the DNA from lily bulbs and shoots were significantly improved by increasing extraction times and using 3 M NaAc,and reduced pollution rate.