基于rDNA ITS序列和RAPD分子标记的桑黄菌遗传多样性分析

Analysis of Genetic Diversity of Different Phellinus Strains Based on rDNA ITS Sequence and RAPD Markers

[目的]分析桑黄菌的遗传多样性。[方法]利用ITS序列分析和RAP／）分子标记技术对16株不同来源的桑黄菌株进行遗传多样性分析。[结果]16株桑黄菌株的ITSl_5．8s-ITS2序列长度在590～714bp，其中5．8S序列最为保守，均为160bp：ITSl长度为220～310bp，ITS2长度为210-245bp。基于ITS序列构建的系统进化树分析结果，同属不同种的桑黄菌株遗传距离较远，对相同种的杨树桑黄一瓦尼木层孔菌进行RAPD分子标记，表明10株瓦尼木层孔菌（Phellinusvaninii）遗传多态性丰富，10株杨树桑黄菌主要分布在我国东北三省的东部山区，区域相对集中，RAPD的聚类分析结果与地域分布差异关系不明显，没有表现出地域性的遗传多样性差异。[结论]试验研究了菌株的分类地位及系统发育进化关系，并首次在桑黄菌物资源库内积累到杨黄分类学地位和遗传多样性的基础信息，对掌握和积累区域桑黄菌物资源的基础信息，并对将来更好地开发利用这一珍稀菌物资源具有重要的学术价值和现实意义．

[Objective] To analyze the genetic divers＇ity of Phellinus strains. [Method] The genetic diversity of 16 different Phellinus strains based on rDNA ITS sequence and RAPD markers were analyzed. [ Result] The results showed that the length of ITS1 -5.8S -ITS2 was between 590 bp and 714 bp, and the 5.8S sequences of these 16 strains were all 160bp, very conserved. The length of ITS1 was between 220 bp and 310 bp, the length of ITS2 was between 210-245 bp. The phylogenetic analysis 〈javascript：showjdsw（ ＇ showjd_0＇ , ＇j_0＇ ） 〉 based on rDNA and RAPD showed that the genetic distance between different species of Phellinus was distant, the ten strains of Phellinus vaninii had a rich polymor- phism based on RAPD, the relationship between distribution and genetic distance was low. [ Conclusionl Tile relationship between axonomic sta- tus of strains and phylogenetic evolution was studied, which has significant academic value and practical meaning for better utilization and devel- opment of Phellinus stains.