慢性乙型肝炎病人外周血树突状细胞对HBsAg的提呈作用

Antigen presenting role of dendritic cell proliferated from peripheral blood monocytes in patients with chronic hepatitis B

目的：研究慢性乙型肝炎（乙肝）病人外周血培养的树突状细胞（dendritic cell，DC）对HBsAg的提呈作用。方法：从乙肝病人外周血中培养扩增DC，以不同浓度的HBsAg与DC共同孵育1.5h，然后再与10倍的自体Ｔ细胞混合培养5d，结束培养前13h加入37kBq/孔（1μCi＝37kBq）3H胸腺嘧啶。收获细胞，用γ液体闪烁计数仪测定cpm。 结果：抗原处理的DC刺激Ｔ细胞增殖效应（cpm）明显高于未经抗原处理的DC。 结论：从慢性乙肝病人外周血培养的DC对HBsAg有较强的提呈作用。

Objective：To study the presenting effect of dendritic cell (DC) proliferated from peripheral blood monocytes in patients with chronic hepatitis B on HBsAg. Methods：DC proliferated from patient's peripheral blood monocytes by adding GM-CSF and IL-4 were incubated with HBsAg for 1.5h at different concentrations, then mixed with autologous T cell and cocultured in 96-well flat-bottomed microtiter plates for 5d at 37℃ and 5% CO2. 3H-TdR was added 13h before the culture completion, and finally the cells were gathered and the cpm was tested. Results：The proliferation of T cell by DC treated by HBsAg is significantly higher than untreated. Conclusion：DC cultured from peripheral blood monocytes in patients with chronic hepatitis B plays a role in presenting HBsAg .