RP-HPLC法测定止痛化瘀搽剂中欧前胡素和细辛脂素的含量

Determination of Imperatorin and Asarinin in Eliminating Pain and Stasis Liniment by RP-HPLC

目的：建立止痛化瘀搽剂中欧前胡素和细辛脂素的含量测定方法，为其质量标准制定奠定基础。方法：Kromasil C18柱（4．6mm×250mm，5μm），欧前胡素含量测定流动相为：甲醇-水（65：35），流速为1．0mL／min，检测波长为300nm；细辛脂素含量测定流动相为：乙腈-水，梯度洗脱，流速为1．0mL／min，检测波长为287nm.结果：欧前胡素在25．0—375ng范围内呈良好的线性（r=0．9997），加样回收率为98．96％（n=6），RSD为2．28％；细辛脂素在74．0—1110ng范围内呈良好的线性（r=0．9996），加样回收率为96．12％（n=6），RSD为2．20％。结论：该法操作简便，专属性强，重现性好，可为止痛化瘀搽剂质量标准的制定提供科学依据。

Objective：To establish a determination method for imperatorin and asarinin in Eliminating Pain and Stasis Liniment, so as to lay a foundation for its quality standards. Methods ： The column was Kromasil C18 （4. 6 mm × 250 mm, 5 μm）. The mobile phase for determination of imperatorin was methanol - water （65 ： 35 ）, the flow rate was 1.0 mL/ min, and the detective wavelength was 300 nm. The mobile phase for determination of asarinin was acetonitrile -water, gradient elution, the flow rate was 1.0 mL/min, and the detective wavelength was 287 nm. Results ： The linear range of imperatorin and asarinin were 25.0 - 375 ng and 74.0 - 1110 ng respectively. The average recoveries were 98.96% （ n = 6） and 96.12% （n =6）,with RSI）=2.28% and RSD =2.20%. Conclusion：The method is simple operation,highly specific with good reproducibility. This method can provide scientific basis for the quality standards formulating of Eliminating Pain and Stasis Liniment.