手足口病患儿外周血单核细胞亚群的研究

Reaserch on peripheral blood monocyte subpopulation in children with HFMD

目的探讨未经治疗和随访1、3、5 d手足口患儿外周血单核细胞亚群的变化。方法流式细胞仪检测104例未经治疗手足口患儿(包括阴性患儿62例,EV71阳性患儿37例,CVA16阳性患儿5例)和随访1、3、5 d的患儿外周血单核细胞CD14highCD16-亚群、CD14highCD16+亚群和CD14lowCD16+亚群。结果与健康儿童组相比,手足口患儿CD14highCD16+单核细胞亚群所占总单核细胞比例明显升高(t=4.092,P<0.001);CD14highCD16-亚群比例明显降低。阴性、EV71型、CVA16型患儿之间CD14highCD16-亚群、CD14highCD16+亚群和CD14lowCD16+亚群差异均无统计学意义。结论未经治疗的手足口患儿外周血单核细胞亚群的变化可能与EV71及CVA16病毒的持续感染有关,与病毒种类相关性不明显。

Objective To analyze the changes in peripheral blood monocyte subpopulation in children with hand-foot-and-mouth disease （HFMD）. Methods Flow cytometry was used to detect CD14highCD16-, monocyte subpopulations in peripheral blood from 104 children with untreat HFMD, including 62 cases of latent HFMD, 37 cases of EV71 HFMD and 5 cases of CVA16 HFMD, as well as 25 normal children control. Result Compared with the normal control, the proportion of CD14highCD16＋monocytes among total monocytes was significantly elevated （4.0% ± 2.4% vs 9.1% ± 5.4%, t = 4.092, P 〈0.001）, while that of CD14highCD16-monocytes was down-regulated （91.5% ± 5.3% vs 84.7% ± 9.8%,t = 3.070,P〈 0.01） in HFMD. No statistical difference was observed in the proportion of CD14highCD16-, CD14highCD16＋ and CD14lowCD16＋ monocytes among the children with EV71, CVA16 HFMD and those with latent HFMD （t 〉1.062, all P 〉 0.05）. Conclutions The changes in peripheral blood monocyte subpopulation in children with untreat HFMD may be associated with the permanent infection of enterovirus 71 or Coxsackie virus A16, but have no obvious correlation with the types of virus.