胃癌患者血清p53蛋白抗原的检测及其临床意义

Detection of p53 protein in sera from patients with gastric carcinoma and evaluating its clinical significance

目的 评价血清 p5 3蛋白测定对胃癌诊断的价值。方法 采用 P1 80 1 和 DO- 7两株抗 p5 3蛋白 Mc Ab建立了双抗体夹心式 EL ISA,并对胃癌和非胃癌患者血清进行了 p5 3蛋白含量测定。结果 95例胃癌组血清 p5 3蛋白含量为 (14.6± 8.2 5 ) U/L,显著高于 82例非胃癌组的 (6 .0 8± 4.11) U/L,P<0 .0 1。若以大于阴性 x+2 s以上为阳性 ,胃癌组阳性率 33.7% (32 /95 ) ,非胃癌组 3.7% (3/82 ) ,P<0 .0 1。11例血清 p5 3阳性患者免疫组化均呈阳性 ,均以 p5 3- Ig G免疫复合物形式存在 ,对 5例胃癌血清动态观察表明术后 1周 p5 3蛋白仍为阳性 ,术后 1个月显著下降 ,并于术后 3个月消失。结论 血清 p5 3蛋白是胃癌的一个独立的生物学指标。

Objective To evaluate the diagnostic value of p53 protein level in serum from patient with gastric carcinoma.Methods Sera from gastric carcinoma and non gastric carcinoma groups were determined quantitatively for p53 protein with a sandwich ELISA employing two purchased MoAbs P1801 and Do-7.Results The concentration of p53 protein in 95 gastric carcinoma subjects was (14.6±8.25) U/L,which was remarkably increased as compared with (6.08± 4.11 ) U/L in 82 non gastric carcinoma subjects( P <0.01).Thirty two positives out of 95 cases (33.7%) were found in gastric carcinoma compared with 3.7% (3/82) in non gastric carcinoma when surpass negatives mean+2s was believed to be as a positive diagnostic standard( P <0.01).Further studies showed that all cases with positive immunohistochemical staning were found in 11 patients with serum p53 protein.Moreover,there existed p53 IgG immunocompound in these patients.These results implied that the entity type of p53 might contribute to prolong its halftime in peripheral circulation.Conclusion serum p53 protein might be an independent biological mark in diagnosis of gastric carcinoma.