栀子苷在乙腈水体系中诱导相分离分配行为

Partition Characteristics of Geniposide in Inducing Phase Separation of Acetonitrile Aqueous Solution System

研究了栀子乙腈水提取液中的栀子苷在诱导相分离后在两相中的分配行为,建立了乙腈/水分相体系分离纯化栀子苷的新方法。重点考察了能使乙腈/水体系分相的诱导剂的种类,分析了诱导剂的组成比例以及用量、乙腈的体积分数、样品加入量和温度对栀子苷分配行为的影响。当温度为25℃时,栀子的乙腈/水(体积比1∶1)提取液中加入KCl与MgSO4组成的混盐(质量比2∶1)分相诱导剂后,栀子苷的萃取率达到81.63%,含量由分相前的3.05%提高至13.54%。

After geniposide in the acetonitrile aqueous extract solution of Gardenia was separated into two phases by inductors, the partition characteristics of it in two phases were studied. A new method was established for the separation and purification of geniposide. The types of inductors inducing phase separation of acetonitrile aqueous solution were researched in detail. The effects on partition characteristics of geniposide were analyzed via investigation of the composition proportion of inductors, the additive amount of inductors, the volume fraction of acetonitrile, the additive amount of sample and temperature. The results show that when inductors were added into the acetonitrile/aqueous（volume ratio 1∶1） extract at 25 ℃, the preliminary purification of geniposide could be finished by one step. The extraction yield of geniposide reaches 81.63% and the purity is increased from 3.05% primitively to 13.54%. The best inductors are composed of KCl and MgSO4（mass ratio 2∶1）.