酸性铬兰K-OP-牛血清白蛋白体系的共振瑞利散射及共振非线性散射光谱研究及应用

Investigation and application of acid chrome blue K-OP-BSA system by resonance rayleigh scattering and resonance non-linear scattering

在pH3．2的邻苯二甲酸氢钾．HCl缓冲液中，酸性铬兰K（ACBK）一OP与牛血清白蛋白（BSA）形成三元离子缔合物，导致共振瑞利散射（RRS）、二级散射（SOS）和倍频散射（FDS）的显著增强，光谱最大散射波长分别位于420，678和340nm。体系的光散射强度与BSA浓度在一定范围内呈线性增强，RRS在0～3．5mg／L，SOS在0—3mg／L，FDS在0～3mg／L范围内对BSA的检出限分别为0．3，0．7和0．8μg／L，据此建立了测定BSA的共振线性（RRS）和共振非线性光散射（RNLS）分析法。以RRS法考察了酸性铬兰K-OP与白蛋白形成三元缔合物的适宜条件、影响因素等。方法可用于合成样品及血清样品中蛋白含量的测定。

In a buffer solution of potassium hydrogen phthalate-hydrochloric acid （ pH 3.2）, acid chrome blue K （ACBK） and non-ionic surface active agent OP combined with bovine serum albumin （BSA） to form ternary ion- complexes, which resulted in significant enhancement of resonance Rayleigh scattering （ RRS）, Second-Order scattering （SOS） and Frequency-Double scattering （FDS）. The maximum scattering wavelengths were located at 420nm for RRS, 678 nm for SOS and 340 nm for FDS respectively. The light scattering intensity of the system was proportional to the concentration of BSA in certain ranges. The linear ranges were 0 ~ 3.5 mg/L for RRS method, 0 - 3 mg/L for SOS method and 0 ~ 3 mg/L for FDS method, and the detection limits of BSA were 0. 3μg/L （for RRS method）, 0.7 μg/L （for SOS method） and 0. 8 μg/L （for FDS method）, respectively, Basedon the enhancement of light scattering, the determination of BSA by the proposed methods （ resonance linear and nonlinear scattering analysis methods） was established, sensitively. The reaction conditions and influence factors of the ACBK-OP-BSA system were investigated by RRS method. The proposed method can be applied to the determination of protein content in synthetic samples and serum samples.