补肾壮骨冲剂对肠源性5-羟色胺及骨密度的影响

Impact of Bushen Zhuanggu Granules on Enterogenous 5-hydroxytryptamine and Bone Density

目的：观察补肾壮骨冲剂对原发性骨质疏松症患者（Osteoporosis，OP）的肠源性5-羟色胺（5-hydroxytryptamine，5-HT）浓度和骨密度的影响，探讨其作用机制。方法：分析60例原发性骨质疏松症患者，在服用补肾壮骨冲剂治疗前后肠源性5-HT浓度的差异。1）本研究所有样本为2011年5月至2012年12月间到广州军区广州总医院住院部及门诊部就诊的确诊为原发性骨质疏松症的患者。2）肠源性5-HT测定：上午8～9点抽取禁食12 h后空腹肘静脉血3 mL，3000 r/min即时离心5 min后，将血清置于-80℃冰箱保存，采用酶联免疫分析法检测5-HT的含量。3）骨密度测定：采用美国GE公司生产的Lunar Prodigy双能X线（ DEXA）骨密度仪，检测各部位BMD。4）骨生化指标测定：采用酶联免疫分析法检测血清中肠源性5-HT的含量、电化学发光免疫分析法分析血清P1NP、β-Crosslaps、PTH的含量。结果：治疗半年后血清中的5-HT浓度下降5.20％、PINP浓度下降12.72％、β-Crosslaps下降26.92％、PTH下降5.42％，治疗前后的差异具有统计学意义（ P均＜0.05，表2）；腰椎、左侧股骨颈、Ward’ s三角、大转子和左侧股骨上端的BMD在治疗后分别增加了0.96％，1.67％，2.91％，1.44％和1.29％，与治疗前相比，左侧股骨颈、Ward’s三角和左侧股骨上端处的差异具有统计学意义（P均＜0.05，表3）。结论：补肾壮骨冲剂可以通过调节脾肾功能，减低肠源性5-HT浓度，提高骨密度。

Objective：To analyze the impact of Bushen Zhuanggu Granules on enterogenous 5-hydroxytryptamine and bone density and mechanism of action .Methods：To compare the enterogenous 5-HT levels of 60 osteoporosis patients pre-and post taking Bushen Zhuang-gu Granules.1）All the patients enrolled were diagnosed as osteoporosis , who were in-patients or out-patients of the General Hospital of Guangzhou Command between May 2011 to December 2012.2） Measureing enterogenous 5-HT：drew elbow venous blood 3ml between 8-9 am （fasting）, centrifuged at 3000 r/min for 5 minutes at room temperature, and immediately frozen at-80℃.5-HT levels were meas-ured by ELISA.3）Bone density was measured using GE company＆#39;s Lunar Prodigy dual energy X ray（DEXA）bone density meter.4） Bi-ochemical indices of bone：PINP、β-Crosslaps and PTH levels were measured using electrochemiluminescence immunoassay .Results：Af-ter 6-months of treatment , the 5-HT levels were reduced by 5.20%, the levels of PINP、β-Crosslaps、PTH were respectively reduced by 12.72%、26.92%and 5.42% （ P〈0.05）; the BMD of lumbar spine L1-L4, femoral neck, wards and troch were increased by 0. 96%、1.67%、2.91%、1.44%and 1.29%（P〈0.05）.Conclusion：The Bushen Zhuanggu Granules can adjust the spleen and kidney functions , decrease the level of enterogenous 5-HT, and increase bone density .