负压封闭引流对猪软组织金黄色葡萄球菌感染创面愈合的影响

Effect of vacuum sealing drainage on healing of staphylococcus aureus-infected wounds in soft tissues of pigs

目的 观察负压封闭引流时负压值的大小对猪皮肤软组织感染金黄色葡萄球菌创面生长的影响.方法 猪皮肤软组织感染金黄色葡萄球菌创面模型,样本共32个,分别给予1 ～32编号,采用随机数字表法将32个创面模型随机分为4组,每组8个样本.负压值-10、-25、-50 kPa为治疗组,常规换药为对照组.作用时间均为72 h.取治疗前后创面中心及周围脓液稠厚处的脓液行细菌计数,并观察创面的一般情况.结果 与常规换药组比较,细菌计数在负压值为-25 kPa时明显减少（P＜0.01）,分泌物减少,黄色脓液组织明显减少,肉芽组织新鲜,创面明显缩小,伤口周围无组织水肿,愈合时间缩短,伤口愈合好.结论 负压封闭引流技术能抑制金黄色葡萄球菌的生长,但负压值为-25 kPa时更能有效减少创面黄色脓液分泌物的产生,抑制金黄色葡萄球菌的生长增殖,刺激新鲜肉芽组织的生成,缩短创面愈合的时间.

Objective To study the effect of negative pressure of vacuum sealing drainage （VSD） on the healing of Staphylococcus aureus-infected wounds in the soft tissue of pigs.Methods Thirty-two Staphylococcus aureus infection models of skin and soft tissue wounds were established,and randomly and equally divided into four groups as control group （with conventional dressing） and three VSD treatment groups according to the negative pressure as-10,-25,and-50 kPa of drainage respectively.Pre-and post-treatment biopsy was performed from the center of the wounds for pathologic observation and bacteria counting.Results As compared with VSD groups,the wound in the control group（P 〈 0.01） got higher bacteria counts,more secretions,more yellow colored tissues,obvious edema around the wound,and longer healing time.Conclusion Degative pressure of VSD can reduce the secretion of the wound,inhibits the growth and proliferation of Staphylococcus aureus,stimulate the formation of granulation tissue and thus shorten the time for wound healing.