甘蔗宿根萌发期內源激素变化与其宿根性的关系

Relationship between the Ratoon Ability and the Change of Endogenous Hormone in Sugarcane at Sprouting Stage

采用酶联免疫法和茎基部取样的方式,以强宿根甘蔗品种‘新台糖22号’为对照,通过田间试验研究了同一组合(‘粤糖97-20’×ROC25)杂交后代分离产生的强宿根甘蔗品(种)系(‘云蔗06-407’、‘云蔗06-415’)和弱宿根甘蔗品(种)系(‘云蔗06-408’、‘云蔗06-416’)宿根萌发期内源激素含量的变化与甘蔗宿根性的关系。结果显示:(1)宿根萌发过程中,强宿根与弱宿根甘蔗茎基部IAA含量、ZR含量和ZR/IAA比值的变化波动大,但差异不显著。(2)强宿根与弱宿根甘蔗茎基部GA3和ABA含量、IAA/ABA和GA3/ABA比值的变化差异较大,其中ABA含量、IAA/ABA和GA3/ABA比值的差异显著,是与甘蔗宿根性关系密切的3个重要参数。(3)强宿根甘蔗ABA含量高于弱宿根甘蔗,而其IAA/ABA和GA3/ABA比值小于弱宿根甘蔗,且IAA/ABA和GA3/ABA比值随甘蔗宿根性的减弱而增大。研究认为,在宿根萌发期,甘蔗宿根性越强,ABA含量越高,IAA/ABA和GA3/ABA比值越小;而这3个重要参数均与ABA有关,进行甘蔗宿根性评价应着重参考ABA含量,同时结合IAA/ABA和GA3/ABA比值再进行深入分析鉴定。

During the sprouting stage in ratoon crop of sugarcane, with the methods of ELISA and bottom part sampling,we studied the relationship of changes in endogenous hormone between good （＇CYZ06-407＇ and ＇ CYZ06-415 ＇） and poor （＇ CYZ06-408 ~ and ＇ CYZ06-416＇） ratoonability sugarcane lines generated from one family ＇CYT97-20＇ X ROC25 ,where,good ratooning sugarcane cultivar ROC22 was used as con- trol. The result indicated that： （1）The changes in content of IAA,content of ZR and the ratio of ZR/IAA in bottom part of stalk for good/poor ratooning lines were strong during sprouting stage. No significant difference were found between good and poor ratooning lines. （2）At bottom parts of cane stalk, the changes in content of GA3, content of ABA, ratios of IAA/ABA and ratios of GA3/ABA between good and poor ra- tooning lines differed significantly. The most significant difference were found in content of ABA,ratios of IAA/ABA and ratios of GA3/ABA, which were important parameters~ （3）Higher content of ABA in good ratooning lines than that in poor ones,lower ratios of IAA/ABA and ratio of GAa/ABA than that in poor ones,with the declines in ratoonability,the ratios of IAA/ABA and GA3/ABA increased. The result sug- gested good ratoonability was characterized high content of ABA and low ratios of IAA/ABA and GA3/ ABA,among which, content of ABA is the most important parameter for evaluating ratoonability. Meanwhile,other two important parameters IAA/ABA and GA3/ABA ratios were recommended as well.