补肾益冲抗衰汤对卵巢储备功能下降大鼠卵巢颗粒细胞凋亡因子Bcl-2、Bax、Caspase-3的影响

The Effect of Bushenyichongkangshuai Decoction on Ovarian Granule Cell Apoptosis Factors Bcl-2,Bax,Caspase-3 of DOR Rat Model

[目的]观察补肾益冲抗衰汤对卵巢储备功能下降模型大鼠卵巢颗粒细胞凋亡因子Bcl-2、Bax、Caspase-3蛋白表达的影响。[方法]以70只8周龄雌性SD大鼠为研究对象,采用雷公藤多苷片制作卵巢储备功能下降大鼠模型。观察大鼠一般情况、卵巢指数、卵巢组织病理形态、血清E2、FSH、FSH/LH水平及卵巢颗粒细胞凋亡因子Bcl-2、Bax、Caspase-3蛋白表达。[结果]补肾益冲抗衰汤能使卵巢储备功能下降大鼠动情周期缩短、子宫、卵巢重量增加,大鼠血清FSH水平、FSH/LH降低,血清E2水平增高(P<0.05或P<0.01),使卵巢颗粒细胞抑制细胞凋亡因子Bcl-2蛋白表达增加,促进细胞凋亡因子Bax、Caspase-3蛋白表达减少(P<0.05或P<0.01)。[结论]雷公藤多甙片致DOR大鼠模型与人类卵巢储备功能下降的临床表现基本一致。细胞凋亡调节因子Bcl-2、Bax、Caspase-3蛋白与卵巢储备功能密切相关,补肾益冲抗衰汤有抑制卵巢颗粒细胞凋亡、减少卵泡闭锁从而改善卵巢储备功能的作用。

[Objective]To investigate the effect of Bushenyichongkangshuai decoction on ovarian granule cellapoptosis Bcl-2, Bax, Caspase-3 of DOR rat modeled by Tripterygium. [Methods]8-week old 70 healthy female rats as the research object ,rat models were made of Tripterygium,the rats estrus cycle, fol icle stimulating hormone（FSH）, luteinizing hormone（LH）, estrogen（E2）, the morphological changes of the ovary and uterus, ovarian granule cellapopto-sis factors Bcl-2, Bax and Caspase-3 were detected. [Results] Bushenyichongkangshuai can increase emotional cycles, uterine, ovarian weight, change serum FSH, FSH/LH, E2（P〈0.05 or P〈0.01）,and impact ovarian granule cells apoptosis factors Bcl-2, and Bax in caspase-3 has significant differences（ P〈0.05 or P〈0.01）. [Conclusion] The diminished ovarian reserve model rats set up with Tripterygium wilfordii is corresponding to DOR typical performance of human.Apoptosis regulatory factors Bcl-2, Bax, caspase-3 protein are closely related to ovarian reserve, BSYCKSD can improve DOR sex hormone lev-els in rats,reduce the fol icular atresia and strengthen the function of ovary .