肉桂配方颗粒的HPLC指纹图谱研究

Study on HPLC Fingerprint of Cinnamomum cassia Formula Granules

目的:建立肉桂配方颗粒的指纹图谱。方法:采用反相高效液相色谱法。色谱柱为Thermo Hypersil ODS-2(250 mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液(28∶72,V/V),检测波长为280 nm,流速为1.0 ml/min,进样量为10μl。采用《中药色谱指纹图谱相似度评价系统软件(2004A版)》对10批样品进行相似度评价。结果:10批肉桂配方颗粒共检出4个共有指纹峰,并指认出其中桂皮醛、肉桂酸、香豆素3个化学成分。10批样品指纹图谱的相似度均>0.900。结论:所建指纹图谱重复性好,可作为肉桂药材及其配方颗粒的质量控制方法。

ABSTRACT OBJECTIVE： To establish the fingerprint of Cinnamomi cassia formula granules. METHODS： RP-HPLC method was adopted. The determination was performed on Thermo hypersil ODS-2 （250 mm× 4.6 mm, 5μm） column with acetonitrile-0.1% phosphoric acid（28 ： 72, V/V） at the flow rate of 1.0 ml/min. The detection wavelength was set at 280 nm and sample size was 10 txl. The similarity of 10 batches of samples were evaluated by using Similarity Calculation Software of TCM Chromatogram Fingerprint （2004 A edition）. RESULTS： 4 common peaks were identified in 10 batches of samples, and 3 chemical compounds were identi- fied, such as cinnamyl dldehyde, cinnamic acid, coumarin. The similarity of fingerprint were all higher than 0.900. CONCLUSIONS： HPLC fingerprint is reproducible, and a suitable method for quality control of C. cassia and C. cassia formula granules.