淇河鲫不同组织器官溶菌酶与抑菌活性比较

Comparison of Lysozyme and Antibacterial Activity among Different Tissues and Organs of Carassius auratus in Qihe River

选择天然三倍体淇河鲫(Carassius auratus in Qihe River),通过试验研究其不同组织器官溶菌酶和抑菌活性,以了解淇河鲫不同组织器官的抗菌能力。结果表明,淇河鲫不同组织器官溶菌酶和抑菌活性存在差异,溶菌酶活性测定值依次为:肝胰脏>肾脏>鳃>脑>肠>脾脏;抑菌活性测定值依次为:肝胰脏>肾脏>脑>鳃>肠>脾脏(P<0.01);淇河鲫抗菌活性较高的组织为肝胰脏和肾脏,且肝胰脏又极显著高于肾脏(P<0.01),肾脏和鳃的抗菌能力极显著高于脾脏(P<0.01);表明鱼类不同组织器官免疫能力和抗菌活性存在差异,这种差异与组织器官的生理功能和抗病响应机制不同有关;同时说明鱼类不同组织器官在致病菌干预过程中将发挥不同的作用。

Carassius auratus in Qihe River, a natural triploid crucian carp, is an important commercial fish. To understand its antibacterial ability in various tissues and organs will be useful for preventing disease. In this study, lysozyme and antibacterial activities were measured in different tissues and organs of C.auratus in Qihe River. The results showed that lysozyme and antibacterial activities in various tissues and organs were different. The measured values of lysozyme activities were： hepatopancreas〉 kidney〉 gill〉brain〉 intestine〉 spleen. Antibacterial activities were shown by the order of hepatopancreas〉 kidney〉brain〉gill〉 intestine〉 spleen, with significant difference among them （P〈0.01）. Antibacterial activities in hepatopancreas and kidney were significantly higher than those in other tissues and organs, and the activity was significantly higher in hepatopancreas than in kidney （P〈0.01）. The antibacterial ability in kidney and gill was significantly higher than that in spleen （P〈0.01）. The research indicates there is difference in lysozyme and antimicrobial activity among the various tissues and organs of fish, which is resulted from the different physiological function of various tissues and organs. At the same time, it is suggested that different tissues and organs play different roles in the intervention process of pathogenic bacteria in fish.