VE-cadherin在不同侵袭性能非小细胞肺癌细胞株的表达及沉默VE-cadherin对非小细胞肺癌细胞株侵袭、迁移的影响

Expression of VE-cadherin in different non-small cell lung cancer cell lines and effects of VE-cadherin silence on the invasion and metastasis of nonsmall cell lung cancer cell lines

目的:探讨VE-cadherin在不同侵袭性能非小细胞肺癌(non-small cell lung cancer,NSCLC)细胞株的表达及沉默VEcadherin对NSCLC细胞株侵袭、迁移的影响。方法:通过定量PCR及Western blot检测VE-cadherin在高侵袭性能的NSCLC细胞株PG、中度侵袭性能的NSCLC细胞株H1299及低侵袭性能的NSCLC细胞株PA中mRNA及蛋白的表达;并通过RNA干扰沉默VE-cadherin的表达来探讨其对NSCLC细胞株PG侵袭、迁移能力的影响。结果:VE-cadherin在3种NSCLC细胞株中都有表达,且其mRNA及蛋白在3种细胞株中的表达强度依次为PG、H1299、PA,PG与H1299相比较,差异有统计学意义(P=0.000),PA与PG、H1299相比较,差异均具有统计学意义(P=0.000);Transwell小室基质侵袭实验结果显示,干扰组与对照组的侵袭细胞数分别为(34.33±3.06)个和(86.00±7.93)个,组间比较差异有统计学意义(P=0.000);划痕损伤实验结果显示,对照组与干扰转染组的细胞迁移距离相比,24 h内没有明显差异(P=0.825),而48、72 h内对照组明显高于干扰转染组,差异具有统计学意义(P=0.000)。结论:VE-cadherin可能参与NSCLC的侵袭和迁移,并在此过程中起重要作用;VE-cadherin可能会成为治疗NSCLC侵袭、血管生成和转移的又一新靶点。

Objective ：To detect the expression of VE-cadherin in different non-small cell lung cancer cell（NSCLC） lines with distinct invasion performance and to investigate the effects of the VE-cadherin silence on the invasion and metastasis of NSCLC cell lines. Methods：Expression of the VE-cadherin mRNA and protein were detected by quantitative PCR and Western blot in NSCLC cell lines with highly invasive, normally and low invasive ability （PG, H 1299 and PA）. Effects of expression of VE-cadherin on the invasion and metastasis of PG cell line were explored by RNA interference. Results：VE-cadherin expressed in 3 kinds of NSCLC cell lines. mRNA and protein expression intensity of VE-cadherin was higher in PG,followed by H1299 and PA cell lines with statistical differ- ences between PG and H1299 （P=0.000） and statistical differences between PA and H1299,PG（P=0.000）. Result of Transwell chamber matrigal invasion assays revealed that the numbers of invasive cells of control group and interference group were 34.33±3.06 and 86.00± 7.93 respectively,with statistical differences between groups （P=0.000）. Results of scratch damage experiment showed that the migration abilities between control group and interference group had no obvious differences in the first 24 h（P= 0.825 ）. But after 48 h or 72 h,metastasis distance of control group was farther than that of interference group with statistical differences （P=0.000）. Invasion and metastasis of PG cell were down regulated by the reduction of the expression of VE-cadherin. Conclusions ： VE-cadherin may play a critical role in the invasion and metastasis of NSCLC and may become a new target in the prevention and treatment of invasion, angiogenesis and metastasis of NSCLC.