鸭胸腺细胞自然凋亡的电镜观察

ELECTRON MICROSCOPIC OBSERVATIONS ON APOPTOSIS OF THYMOCYTES IN THE DUCK THYMUS

应用透射电镜观察了鸭胸腺细胞自然凋亡的超微结构变化。结果表明 ,胸腺细胞发生凋亡时 ,表现为染色质凝聚、边集 ,细胞核呈现新月形、“C”形、花瓣状、圆环状或圆斑状 ;粗面内质网、线粒体、溶酶体先是大量增生 ,之后粗面内质网包裹各种细胞成分形成自噬体并与溶酶体融合 ,再逐渐演变成凋亡小体 ;线粒体在凋亡小体内发生肿胀和空泡化 ;最后 ,凋亡细胞及凋亡小体被巨噬细胞吞噬、清除。

The apoptosis of thymocytes in the duck thymus was observed under transmission electron microscope.The results revealed that the apoptotic thymocytes underwent a series of morphologic changes:the chromatin condensed and marginated,and the nuclei appeared crescent,“C” shaped,petal like,ring like,or dark round;the rough endoplasmic reticula,mitochondria and lysosomes first proliferated in large numbers,then the rough endoplasmic reticula separated and wrapped various elements of the apoptotic thymocytes to form autophagosomes,which fused with the lysosomes and became gradually apoptotic bodies;the mitochondria in the apoptotic bodies swelled and vacuolized;and finally the apoptotic thymocytes and apoptotic bodies were phagocytosed and eliminated by thymic macrophages.