电子顺磁共振对五种中草药抗氧化活性的研究

Study on Antioxidant Activities of Traditional Chinese Medicines by Electron Paramagnetic Resonance Technique

采用电子顺磁共振(EPR)技术,通过大黄、香青兰、香茅草、懈寄生和骆驼蓬子五种中草药对1,1-二苯基-2-苦基肼自由基(DPPH)、羟基自由基(·OH)和超氧阴离子自由基(O-·2)3种自由基清除能力大小的比较,研究了大黄等五种中草药抗氧化活性能力。这五种中草药对3种自由基均有一定的清除作用,其中大黄清除DPPH和超氧阴离子自由基的IC50小于0.5 mg/mL,清除羟基自由基的IC50=1.892 mg/mL。结果表明五种中草药中大黄的抗氧化活性能力最强,为中草药用于生物体的抗氧化应激研究提供依据。

In this study, electron paramagnetic resonance （EPR） technique was used to investigate the antioxidant activities of five traditional Chinese medicines （TCM）, including rhubarb ,Dracocephalum moldavica L. , lemongrass, mistletoe and seed of common peganum. The 1,1- diphenyl -2- picryl-hydrazyl radical （ DPPH）, hydroxyl free radical and superoxide anion free radicals scavenging abilities of the five studied TCMs were investigated. The results indicated that the five studied TCMs all showed scavenging abilities against three free radicals,in which rhubarb exhibited the strongest activities. Its IC50 of scavenging DPPH and superoxide anion free radical were all less than 0.5 mg/mL and the IC50 of scavenging hydroxyl free radical was 1. 892 mg/mL.