摘要
以油菜无菌苗切取下胚轴预培养4d后，直接进行不同的剂量的γ射线辐射处理，结果显示，10～30Gyγ射线辐射可明显促进外植体的再生，取下胚轴诱变后的再生苗种植田间，对M1代农艺性状进行考察，结果表明下胚轴辐射超过60Gy剂量的照射，存在较大的变异。
The hypocotyls of 7 days seedling age were treated with γ-rays irradiation with diferent dose after precultured for 4 days in the precultureed media.Results showed that the regeneration frequency of hy-pocotyls could be increased by irradiation treatment ofγ-rays with the lower dose of 20Gy to predultured hypocotyls.The explants of irradiated hypocotyls were planted in the field,and observed the agriculture char-acters of M1 generation. Results showed that the effect to agriculture characters is significant by irradiation treatment of γ-rays with higher than 60Gy dose to preculture hypocotyls.
出处
《安徽农学通报》
2014年第6期31-32,共2页
Anhui Agricultural Science Bulletin
