超微粉碎技术对三七药材粉碎效果及有效成分含量的影响

Influence of particle sizes and content of effective compositions of Panax notoginseng powders crashing by superfine somminution technique

该实验主要研究了超微粉碎技术对三七药材粉碎效果及有效成分含量的影响。以三七微粉粒径大小为指标,利用马尔文粒径测定仪测定粉碎不同时间所得到三七微粉的粒径并采用HPLC-ELSD对三七超微粉碎前后,人参皂苷Rg1,Rb1和三七皂苷R1的含量进行了测定。结果显示超微粉碎2 h的药材粉末粒径均一,达到了细胞级粉碎,D50约为9.599μm,粒径分布为单峰,粉碎0.5,1,1.5 h的粒径分布均为双峰;超微粉碎0.5,1,1.5,2 h所得三七微粉中3种皂苷类成分总量分别为7.7%,7.5%,7.5%,8.3%,普通粉碎所得三七药粉中的3种皂苷总量约为5.0%,通过以上实验可以看出:超微粉碎与否对三七粉粒径均匀度及其主要成分含量有很大影响;与普通粉碎药粉相比,超微粉碎2 h药粉粒径较为均一,3种皂苷含量明显增加,为临床减少三七用药剂量及合理利用资源提供依据;但其皂苷总量与粉碎时间不成正比关系,可以结合粒径大小需求选择不同的粉碎时间。

In this study, superfine comminution technique was applied to destroy the cell wall of the Panax notoginseng, and then the influence of the particle sizes and the content of effective composition of the P. notoginseng powders were learned, comparing with the common powders. Superfine comminution technique was used for 0. 5, 1, 1.5, 2 h, respectively and the particle sizes, unifir- mity were regarded as the evaluation index. Then, the sizes of the powders was measured that were crashed with different time by Malv- ern Mastersizer 2000 ＋ Scricco 2000 and the total content of ginsenoside RgI , ginsenoside Rb1 , notoginsenoside R1 in the superfine powder of P. notoginseng were determined by HPLC-ELSD. Finally, the powder that crashed for two hours possess the more uniform in sizes that is at cell level, Ds0 is about 9. 599 Ixm, the size distribution was presented as one peak, the other three samples were two peaks. The total content of the three saponins in the four samples that crashed for 0. 5, 1, 1.5, 2 h by superfine comminution tech- nique were 7.7% , 7.5% , 7.5% , 8.3%. However, the total content of the three ingredients in the common powder was 5.0%. This investigation indicated that superfine comminution technique has remarkable effect on particle size and uniformity of the common powder of P. notoginseng. By comparing the superfine powder and common powder, it was found that the method obviously improved the total content of the saponins and provided a basis for reducing dosage of notoginseng in clinical application, but the content and the crashed time were not the linear relationship. The crashed time can be chosen by combining with the demand partical sizes in the production.