摘要
目的 :证实人牙乳头细胞中 BMP- 7的表达 ,获得 h BMP- 7成熟肽基因。方法 :提取人牙乳头细胞总 RNA,反转录合成 c DNA,以特异性引物扩增 h BMP- 7成熟肽基因并克隆入 T载体 ,筛选阳性克隆进行序列测定。结果 :PCR扩增得到一特异性的约 430 bp的片段 ,克隆后筛选出阳性克隆 ,序列测定结果表明与国外已发表的序列完全一致。结论 :人牙乳头细胞中有 BMP- 7基因的表达 ,并克隆得到 h BMP- 7成熟肽基因 ,为 h BMP-
?Objective:To clone and sequence the gene encoding hBMP 7 mature peptide from human dental papilla mesenchymal cells. Methods: Total RNA was extracted from cultured human dental papilla mesenchymal cells and the desired cDNA fragment was obtained by RT PCR with two specific primers. The segment was inserted into T vector and the result plasmid was transformed into DH5α.The positive clone was sequenced.Results:A 430 bp specific fragment was obtained.The sequence of the gene was consistent with that encoding hBMP 7 mature peptide in the published references. Conclusion: The gene encoding hBMP 7 mature peptide exists in human dental papilla mesenchymal cells.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2001年第1期3-5,共3页
Journal of Practical Stomatology