摘要
目的对小鼠心肌缺血再灌注损伤模型制备及心肌梗死面积评价方法进行比较。方法40只雄性BAB/c小鼠按完全随机设计方法分为传统方法组和改良方法组,传统方法组的心肌再灌注操作采用再次开胸、松开结扎线的方法,改良方法组的心肌再灌注操作为在体外松开结扎线,无需再次开胸。采用伊文思蓝-TTC双染色方法区分缺血再灌注损伤后心肌的梗死区(IA)、缺血危险区(AAR)和左心室总面积(LV)。同时采用传统算法和质量权重法计算心肌梗死面积,质量权重法即在传统算法的基础上,用切片中各区域面积乘以该片心肌质量的百分比,累加各切片的相应区域面积,再计算百分比。结果无论采用哪种算法,传统方法组与改良方法组心肌梗死面积(IA/AAR)差异无统计学意义(传统算法:传统方法组44.43%±2.28%,改良方法组44.24%±1.68%,P=0.96;质量权重法:传统方法组51.74%±2.26%,改良方法组54.51%±1.14%,P=0.23)。与传统方法组相比,改良方法组术后存活率高、麻醉剂使用剂量小、苏醒时间短(均为P<0.05)。此外,采用质量权重法计算AAR/LV、IA/AAR数据的标准差显著小于传统算法(AAR/LV:传统算法标准差2.90,质量权重法标准差1.24;IA/AAR:传统算法标准差2.22,质量权重法标准差:1.00)。结论与传统方法相比,改良方法能够更加稳定、有效地制备小鼠心肌缺血再灌注模型。此外,采用质量权重法计算的心肌梗死面积数据比传统方法准确性更高。
Objective The study aims to establish an efficient mouse model of myocardial ischemia/reperfusion and the assessment of myocardial infarct size. Methods Forty male BAB/c mice were equally divided into the classic method group and the new method group. In the classic method group, reperfusion was achieved by reopening the chest and releasing the ligation, while in the new method group reperfusion was achieved by releasing the slipknot without opening the chest for the second time. Infarct area (IA), area at risk (AAR) and left ventricular (LV) area was quantitatively evaluated by Evans blue-TFC staining. Two measurements were applied to analyze the data. Compared to the classic measurement, IA, AAR and LV areas on each section were multiplied by the percentage of weight of the section and then totaled from all sections in new measurement. Results The myocardial infarction size (IA/AAR) was not significantly different between classic and new method group ( classic measurement: classic method group44. 43% ±2. 28% new method group 44. 24% ±1.68% ,P =0. 96;new measurement:classic method group 51.74% ±2. 26% new method group 54. 51% ± 1.14% ,P = 0. 23). Higher survival rate, less anesthetic dosage and reduced analepsia time were observed in the new method group in comparison with the classic method group ( all P 〈 0. 05). In addition, the standard deviation of both AAR/L~ and IA/AAR were lower in the new measurement group. Conclusions The new method for establishing myocardial ischemia/ reperfusion mouse model is more efficient compared with the classic method. Furthermore the new t is more accurate for the assessment of myocardial infarct size than the classic measurement.
出处
《中国心血管杂志》
2014年第2期123-127,共5页
Chinese Journal of Cardiovascular Medicine