严重烧伤对大鼠肠三叶因子表达的影响

The changes of intestinal trefoil factor expression in rat small intestine after severe burn injury

目的 探讨严重烧伤对大鼠肠三叶因子 (ITF)及其mRNA表达的影响及意义。 方法 48只健康成年Wistar大鼠 ,制成 30 %TBSAⅢ度烧伤模型并随机分成正常对照组及伤后 1,2 ,3,5 ,7d共 6组 ,每组 8只。采用原位杂交、免疫组化等手段观察伤后ITF及ITFmRNA表达的变化 ,并使用高效液相色谱仪对肠黏膜ITF含量进行定量检测。 结果 正常对照组大鼠小肠中ITF及ITFmRNA均有一定表达 ,它们主要分布于肠绒毛杯状细胞中。烧伤后肠黏膜组织结构受损 ,ITFmRNA表达明显减少 ,肠杯状细胞合成和分泌ITF的能力大幅下降 (P <0 .0 1) ,特别是ITF二聚体的含量远远低于伤前 [(36 9.33± 6 5 .5 6 )ng/g],伤后 7d[(15 .83± 4.40 )ng/g]仅为伤前的 4%。 结论 严重烧伤后肠黏膜组织结构受损是ITF合成下降的主要原因 ,而ITF特别是ITF二聚体含量的大幅下降又可加重肠黏膜损伤 ,延缓肠黏膜修复。

Objective To explore the changes of intestinal trefoil factor(ITF) as well as ITF mRNA expression in rat small intestine after severe burn injury . Methods The distribution and the content of ITF in intestine were quantitatively determined with in situ hybridization (ISH), immunohistochemistry and high performance liquid chromatography (HPLC). Results ITF and ITF mRNA were distributed in whole small intestine and most of them localized in goblet cell of intestinal villus. After burn injury, the structure of intestinal mucosa was severely damaged and ITF mRNA expression was lessened. Moreover, the ability of goblet cell synthesis and ITF secretion, especially ITF dimmer were significantly decreased, i.e., from (369.33±65.56) ng/g before burn injury to (15.83±4.40) ng/g 7 days after burn injury, only accounting for 4% of that before injury. Conclusions The post burn damage to intestinal structure is the main cause for the declination in ability of goblet cell synthesis and ITF secretion; and ITF drop especially ITF dimmer can enhance intestinal mucosa injury and delay intestinal mucosa repair.